Acquisition of Circular Dichroism Spectropolarimeter

购买圆二色性分光偏振计

• 批准号: 0618713
• 负责人: Kathleen Mandell
• 金额: --
• 依托单位: Beloit College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-01 至 2009-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0618713&HistoricalAwards=false
• 关键词: Acquisition; Circular; Dichroism; Spectropolarimeter

This award is for the acquisition of a circular dichroism (CD) spectropolarimeter with stopped flow and total fluorescence accessories. This instrument will be used to measure the denaturation transition and conformational unfolding of DNA oligomers and proteins due to confinement effects. The investigators will use the CD instrumentation to complement Differential Scanning Calorimetry and Isothermal Titration Calorimetry for the following uses: to monitor conformational ordering upon DNA duplex formation, to determine whether this parameter is sequence dependent, to measure denaturation process of trinucleotide DNA duplex, and to measure conformational change upon drugs binding to DNA. Acquisition of this CD instrumentation will greatly enhance inquiry-based curriculum in the general chemistry, biochemistry, inorganic chemistry and physical chemistry courses at the college, in addition to its benefits for undergraduate student researchers. Acquisition of this instrumentation also strengthens the collaboration between this undergraduate college and Argonne National Laboratory.

该奖项是为了奖励一台带有停流和全荧光附件的圆二向色性(CD)光谱偏振仪。该仪器将用于测量DNA寡聚体和蛋白质由于限制效应而发生的变性转变和构象展开。研究人员将使用CD仪器来补充差示扫描量热法和等温滴定量热法的以下用途：监测DNA双链形成时的构象有序性，确定该参数是否与序列有关，测量三核苷酸DNA双链的变性过程，以及测量药物与DNA结合时的构象变化。购买这种CD仪器将极大地增强学院普通化学、生物化学、无机化学和物理化学课程的探究式课程，同时对本科生研究人员也有好处。这一仪器的收购也加强了这所本科学院与阿贡国家实验室之间的合作。