Instrumentation for the Enhancement of DRI's Microbial Ecology Education and Research (MEER) Programs

用于加强 DRI 微生物生态教育和研究 (MEER) 计划的仪器

• 批准号: 0421514
• 负责人: Christian Fritsen
• 金额: $ 45.41万
• 依托单位: Nevada System of Higher Education, Desert Research Institute
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-01 至 2007-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0421514&HistoricalAwards=false
• 关键词: Instrumentation; Enhancement; DRI; Microbial; Ecology

The objectives of the proposed instrument acquisition are to enhance the analytical capabilities and benefit ongoing and future research in the field of microbial ecology in extreme environments. The capabilities provided by these instruments will aid in the integration of terrestrial, hydrologic, and atmospheric research within the PIs' university system, address environmental problems associated with microbial ecology, and enhance facilities and research programs for the benefit of undergraduate and graduate students. Research made possible by the acquisition of these instruments includes chemotaxonomic and phylogenetic characterization of Antarctic pack ice microbiota, phylogenetic determinations of Bacteria, Eukarya, and Archea from ancient brine habitats, completion of several microbial genomics projects, evaluation of individual cell phylotypes and activities in ice environments, oceans, lakes, and travertine carbonate materials, evaluation of temporal and spatial shifts in taxonomic and enzymatic activities in freshwater ecosystems, and characterization of microbially mediated iron mineral transformations and elemental isotopic signatures reflective of microbial activity. Upcoming research exploring the relationship between the timing of sea ice formation and the seasonal movements of krill, fish, penguin, and whale migration will be facilitated by the acquisition of instruments capable of quantifying factors that have implications for changes in these movements, including characterizing pigment in microalgae, profiling the bacterial community composition, and determining nutrient status. Quantification of accessory pigments will all for more statistically sound comparisons of microbial ice communities associated with early versus late forming ice. Results of this research will have important implications for prediction of the effects of global climate change on the Southern Ocean ecosystem.The instrumentation requested in the proposal will benefit numerous collaborative projects in the field and in the laboratory. The proposed instrumentation will also lead to significant educational and information contributions at several levels. The instrumentation will provide increased opportunities for training with state of the art equipment at the undergraduate, graduate and post doctoral level, and educational opportunities for younger students. Information from the research will be made publicly available through several outlets, including websites generated by the PIs and their collaborators.

拟议仪器采购的目标是提高分析能力，并有利于极端环境中微生物生态学领域正在进行的和未来的研究。 这些仪器所提供的能力将有助于在PI的大学系统内整合陆地，水文和大气研究，解决与微生物生态学相关的环境问题，并为本科生和研究生的利益加强设施和研究计划。 这些仪器的获得使研究成为可能，包括南极浮冰微生物群的化学分类学和系统发育特征，古盐水栖息地细菌，真核生物和太古代的系统发育测定，完成几个微生物基因组学项目，评估冰环境，海洋，湖泊和碳酸盐材料中的单个细胞生物类型和活动，评价淡水生态系统中分类和酶活性的时空变化，以及表征微生物介导的铁矿物转化和反映微生物活动的元素同位素特征。即将到来的研究探索海冰形成的时间和磷虾，鱼类，企鹅和鲸鱼迁移的季节性运动之间的关系将通过收购能够量化的因素，有影响这些运动的变化，包括表征微藻中的色素，剖析细菌群落组成，并确定营养状态的仪器。 辅助色素的定量将有助于对与早期和晚期形成的冰相关的微生物群落进行统计学上更合理的比较。 这项研究的结果将对预测全球气候变化对南大洋生态系统的影响产生重要影响，提案中要求的仪器将有利于实地和实验室的许多合作项目。拟议的仪器还将在若干级别上产生重大的教育和信息贡献。这些仪器将为本科生、研究生和博士后提供更多的培训机会，并为年轻学生提供教育机会。研究的信息将通过几个渠道公开，包括PI及其合作者创建的网站。