Acquisition of Equipment for Genetic Analyses and Long-Term DNA Storage

购置遗传分析和长期 DNA 储存设备

• 批准号: 0421583
• 负责人: Shelley James
• 金额: --
• 依托单位: Bernice P Bishop Museum
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-01 至 2007-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0421583&HistoricalAwards=false
• 关键词: Acquisition; Equipment; Genetic; Analyses; Long

A grant has been awarded to the Bernice P. Bishop Museum under the direction of Drs. Christopher F. Puttock and Shelley A. James to support the acquisition and maintenance of a DNA genetic analysis system, associated sequence analysis software and hardware, a gel analyzing system and spectrophotometer, and equipment for long-term DNA storage. The Pacific Center for Molecular Biodiversity (PCMB) was established at the Bishop Museum in 2001 to further the museum's mission to address natural science and cultural history questions of Hawai'i and the Pacific region through molecular research. Research productivity has been hindered by instrument availability, prolonged delays due to outsourcing for sequencing and genetic analysis, and the lack of ultra-cold storage. Acquisition of instrumentation through the award will greatly enhance the research capacity and training for the study of the biota of Hawaii and the Pacific region in five key areas: specimen preservation; biodiversity; systematics and ecology; identification and forensic studies; and bio-anthropology. It will allow the integration of modern molecular studies with traditional taxonomic studies of Bishop Museum collections. PCMB will store and provide to researchers aliquots of genetic material of biota that are now rare or otherwise unavailable for molecular analysis, thus maintaining the integrity of Bishop Museum collections. New molecular research techniques will be undertaken, with new protocols being developed in a timely manner unhindered by instrument and outsourcing delays. Support provided by this award will result in increased training and education opportunities for scientists and students, particularly for Hawaiian and Pacific Island native peoples and citizens of Asian-American descent by offering internship and traineeship programs. PCMB will provide research opportunities pertaining to Hawaii and the Pacific to be kept within the Hawaiian Islands, while expanding the use of Bishop Museum collections by local, national and international scientific research communities by providing samples of genetic resources and encouraging the use of the available facilities and equipment. Results of research are made available to scientists and the general public through publication using web-based, electronic and traditional media.

在克里斯托弗·F·普托克博士和谢利·A·詹姆斯博士的指导下，贝尼斯·P·毕晓普博物馆获得了一笔赠款，以支持购买和维护DNA遗传分析系统、相关的序列分析软件和硬件、凝胶分析系统和分光光度计，以及长期储存DNA的设备。太平洋分子生物多样性中心(PCMB)于2001年在主教博物馆成立，以促进该博物馆通过分子研究解决夏威夷和太平洋地区的自然科学和文化史问题的使命。研究生产率受到仪器可获得性、测序和基因分析外包造成的长时间延误以及缺乏超低温储存的阻碍。通过该奖项获得的仪器将极大地加强夏威夷和太平洋地区生物群研究的研究能力和在五个关键领域的培训：标本保存；生物多样性；分类学和生态学；鉴定和法医研究；以及生物人类学。它将使现代分子研究与主教博物馆藏品的传统分类学研究相结合。PCMB将储存和向研究人员提供等量的生物群遗传物质，这些遗传物质现在很稀有或无法用于分子分析，从而保持毕晓普博物馆藏品的完整性。将采用新的分子研究技术，及时开发新的协议，不受仪器和外包延迟的阻碍。该奖项提供的支持将增加科学家和学生的培训和教育机会，特别是通过提供实习和实习生计划，增加夏威夷和太平洋岛屿原住民和亚裔美国公民的培训和教育机会。PCMB将提供与夏威夷和太平洋有关的研究机会，保留在夏威夷群岛内，同时通过提供遗传资源样本和鼓励使用现有的设施和设备，扩大地方、国家和国际科学研究界对主教博物馆藏品的使用。研究成果通过网络、电子和传统媒体的出版物向科学家和公众公布。