SST: Ricin Quantification in Aqueous Media

0426170 Grimes这项研究的目的是开发一种高度敏感的，特异性的蓖麻毒素传感器平台，在适当的知识基础上，可以很容易地扩展到能够同时识别水介质（例如水，血液，血清）中的多种生物毒素或病原体。此外，传感器平台被设计为具有相对低的成本，使得能够实际实施所得到的传感器技术。本研究要回答的基本和关键问题是，质量敏感的磁弹性传感器可以修改，以允许蛋白质生物毒素的定量，这些设备可以被包装成一个小的手持传感器，以帮助快速定量蓖麻毒素浓度？为了回答这些问题，提出了以下具体目标：（1）在基础水平上，研究蓖麻毒素生物毒素与生物识别分子（抗体或GSL）修饰的聚合物薄膜的结合。在初步工作中，研究人员已经对蓖麻毒素和E. coli O157：H7。在这里，他们将确定抗体或GSL的模式生物毒素（蓖麻毒素）将结合，然后将这些识别分子的表面上的聚（乙二醇）水凝胶或自组装单层，并研究模型生物毒素与这些改性表面的结合，（2）开发用于图案化这些生物分子的技术-磁致弹性基底上的改性聚合物凝胶，并研究通过将模型毒素结合到传感器表面上而在磁致弹性材料中引起的共振频率的变化，以及（3）将识别分子改性的磁致弹性传感器和适当的电子器件集成到手持装置中，该手持装置可以容易地用于监测一次性盒内收集的流体样品中的生物毒素含量。虽然该提案中的努力严格集中在蓖麻毒素上，但由此产生的传感器技术可以适用于其他毒素。

0426170GrimesThis research is specifically aimed at developing a highly sensitive, specific ricin sensor platform which, with the intellectual foundation in place, can be readily extended to enable simultaneous identification of multiple biotoxins or pathogens in aqueous media (e.g. water, blood, serum). Also, the sensor platform is designed to be of relatively low cost enabling practical implementation of the resulting sensor technology. The fundamental and critical questions to be answered by this research are, can mass sensitive magnetoelastic sensors be modified to permit the quantification of protein biotoxins, and can these devices be packaged into a small hand-held sensor to aid in the rapid quantification of ricin concentrations? To answer these questions the following specific aims are proposed: (1) investigate, on a fundamental level, the binding of the ricin biotoxin to polymer thin films modified with biorecognition molecules (antibodies or GSLs). In preliminary work the investigators have accomplished this for both ricin and E. coli O157:H7. Here they will identify antibodies or GSLs which the model biotoxin (ricin) will bind, then immobilize these recognition molecules on the surface of poly(ethylene glycol) hydrogels or self-assembled monolayers, and study the binding of the model biotoxins to these modified surfaces, (2) develop techniques for patterning these biomolecule-modified polymer gels on magnetoelastic substrates and study the changes in resonance frequency induced in the magnetoelastic material by binding of the model toxins onto the sensor surface, and (3) integrate the recognition molecule-modified magnetoelastic sensors and appropriate electronics into a hand-held device that can be readily used to monitor biotoxin content in collected fluid samples within a disposable cartridge. While efforts in this proposal are strictly focused on ricin, the resulting sensor technology could be adaptable to other toxins.