Genetic Data Collection Capability for the Romberg Tiburon Center, San Francisco State University

旧金山州立大学 Romberg Tiburon 中心的遗传数据收集能力

• 批准号: 0435033
• 负责人: Cynthia Cohen
• 金额: --
• 依托单位: San Francisco State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-11-01 至 2008-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0435033&HistoricalAwards=false
• 关键词: Genetic; Data; Collection; Capability; Romberg

A grant has been awarded to San Francisco State University (SFSU), under the direction of Dr Cynthia Cohen to provide funds for a capillary DNA analyzer for sequencing and fragment analysis and a real-time PCR machine for the Romberg Tiburon Center (RTC) as part of an ongoing plan to develop the joint-use Molecular Genetics Laboratory for RTC resident and visiting scientists and students. The requested equipment will greatly enhance current capabilities for nucleic acid analysis onsite at RTC and move RTC's molecular capabilities significantly forward to a modern laboratory for marine molecular research at scales of organization from the subcellular to ecosystem. Real-time PCR will allow immediate analysis of gene expression and changes in community composition, and sequencing and fragment analysis capability will open doors to RTC users in ecological and evolutionary genetics, molecular evolution, and phylogenetics. Capillary DNA analyzers and real-time PCR machines are becoming a regular part of modern molecular biological laboratories as their versatility and expediency have been proven in numerous environmental scientific studies. Access to these sophisticated molecular techniques will significantly enhance current work at RTC in microbial ecology, invasion biology, population biology, biological oceanography, symbioses, ecosystem function, and life history studies.

在辛西娅·科恩 (Cynthia Cohen) 博士的指导下，旧金山州立大学 (SFSU) 获得了一笔赠款，为 Romberg Tiburon 中心 (RTC) 购买用于测序和片段分析的毛细管 DNA 分析仪和实时 PCR 机提供资金，作为为 RTC 常驻和访问科学家和学生开发联合使用分子遗传学实验室的持续计划的一部分。所需的设备将大大增强 RTC 现有的现场核酸分析能力，并将 RTC 的分子能力显着提升到现代实验室，进行从亚细胞到生态系统组织规模的海洋分子研究。 实时 PCR 将允许立即分析基因表达和群落组成的变化，测序和片段分析功能将为 RTC 用户在生态和进化遗传学、分子进化和系统发育方面打开大门。毛细管 DNA 分析仪和实时 PCR 仪正在成为现代分子生物实验室的常规组成部分，因为它们的多功能性和便利性已在众多环境科学研究中得到证明。获得这些复杂的分子技术将显着加强 RTC 当前在微生物生态学、入侵生物学、种群生物学、生物海洋学、共生体、生态系统功能和生命史研究方面的工作。