Genetic Data Collection Capability for the Romberg Tiburon Center, San Francisco State University

在Cynthia Cohen博士的指导下，旧金山州立大学（SFSU）获得了一笔赠款，为Romberg Tiburon中心（RTC）提供用于测序和片段分析的毛细管DNA分析仪和实时PCR机，这是为RTC常驻和访问科学家和学生开发联合使用分子遗传学实验室计划的一部分。所要求的设备将大大提高RTC现场核酸分析的现有能力，并将RTC的分子能力显著推进到从亚细胞到生态系统的组织规模的海洋分子研究的现代化实验室。实时聚合酶链式反应（Real-time PCR）将允许对基因表达和群落组成变化进行即时分析，测序和片段分析能力将为RTC用户在生态和进化遗传学、分子进化和系统遗传学方面打开大门。毛细管DNA分析仪和实时PCR仪正成为现代分子生物学实验室的常规组成部分，因为它们的多功能性和方便性已经在许多环境科学研究中得到了证明。获得这些复杂的分子技术将大大加强RTC在微生物生态学、入侵生物学、种群生物学、生物海洋学、共生、生态系统功能和生活史研究方面的现有工作。

A grant has been awarded to San Francisco State University (SFSU), under the direction of Dr Cynthia Cohen to provide funds for a capillary DNA analyzer for sequencing and fragment analysis and a real-time PCR machine for the Romberg Tiburon Center (RTC) as part of an ongoing plan to develop the joint-use Molecular Genetics Laboratory for RTC resident and visiting scientists and students. The requested equipment will greatly enhance current capabilities for nucleic acid analysis onsite at RTC and move RTC's molecular capabilities significantly forward to a modern laboratory for marine molecular research at scales of organization from the subcellular to ecosystem. Real-time PCR will allow immediate analysis of gene expression and changes in community composition, and sequencing and fragment analysis capability will open doors to RTC users in ecological and evolutionary genetics, molecular evolution, and phylogenetics. Capillary DNA analyzers and real-time PCR machines are becoming a regular part of modern molecular biological laboratories as their versatility and expediency have been proven in numerous environmental scientific studies. Access to these sophisticated molecular techniques will significantly enhance current work at RTC in microbial ecology, invasion biology, population biology, biological oceanography, symbioses, ecosystem function, and life history studies.