SGER: Waste Biomaterials for Enzymatic Synthesis

摘要提案题目：SGER：用于酶合成的废弃生物材料提案号：cts -0437544首席研究员：Adrienne T. cooper机构：天普大学本提案的目的是大幅扩展酶催化的应用，以替代化学催化合成的环境友好型替代品。目前阻碍生物催化更广泛应用的障碍部分是纯化酶（如过氧化物酶）的高成本，以及对大量材料的要求。有机溶剂的使用也很普遍。然而，如果废弃的生物材料可以用作酶的来源，将大大降低成本。该项目将探索冻干过氧化物酶的潜在替代品，包括从辣根和大豆中提取的植物过氧化物酶。该研究的关键高风险部分是利用原位提取，并评估提取溶剂对酶和催化活性的影响。水/有机混合物和室温离子液体都将用于提取和合成。3-苯基苯酚的聚合反应将用于评价酶的活性。该研究的更广泛影响包括为环境和经济上可持续的生物催化合成替代品提供新的途径。将通过同行评议的期刊发表。将利用强有力的K-12外展活动。该项目确保代表性不足的群体的积极参与。提取和合成环境的结合将是这一领域的重大进展，可能的工业应用将非常重要。

AbstractProposal Title: SGER: Waste Biomaterials for Enzymatic Synthesis Proposal Number: CTS-0437544Principal Investigator: Adrienne T. CooperInstitution: Temple UniversityThe objective of this proposal is to substantially expand the utility of enzyme catalysis for environmentally benign alternatives to chemical catalysis syntheses. Present barriers to wider applications of biocatalysis stem in part to the high cost of purified enzymes, such as peroxidase, coupled with the requirements for large volumes of materials. Use of organic solvents also is prevalent. However, if waste biomaterials could be used as the source of the enzymes, dramatic cost reductions would be achieved. This project would explore promising potential replacements for lyophilized peroxidases, including plant peroxidases derived from horseradish and soybeans. The key high-risk component of the research is the utilization of in situ extraction with the evaluation of the effects of the extraction solvents on enzymatic and catalytic activity. Both aqueous/organic mixtures and room temperature ionic liquids will be utilized for extraction and synthesis. Polymerization of 3-phenylphenol will be used to evaluate the activity of the enzymes. The broader impacts of the research include new avenues to environmentally and economically sustainable alternatives for biocatalysis synthesis. Publication will be through peer-reviewed journals. A strong K-12 outreach activity will be utilized. The project ensures strong participation of underrepresented groups. The combination of the extraction and synthesis environments would be a major advance in this area, and possible industrial applications would be very significant.