SGER: Waste Biomaterials for Enzymatic Synthesis

SGER：用于酶法合成的废弃生物材料

• 批准号: 0437544
• 负责人: Adrienne Cooper
• 金额: --
• 依托单位: Temple University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-01 至 2006-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0437544&HistoricalAwards=false
• 关键词: SGER; Waste; Biomaterials; Enzymatic; Synthesis

AbstractProposal Title: SGER: Waste Biomaterials for Enzymatic Synthesis Proposal Number: CTS-0437544Principal Investigator: Adrienne T. CooperInstitution: Temple UniversityThe objective of this proposal is to substantially expand the utility of enzyme catalysis for environmentally benign alternatives to chemical catalysis syntheses. Present barriers to wider applications of biocatalysis stem in part to the high cost of purified enzymes, such as peroxidase, coupled with the requirements for large volumes of materials. Use of organic solvents also is prevalent. However, if waste biomaterials could be used as the source of the enzymes, dramatic cost reductions would be achieved. This project would explore promising potential replacements for lyophilized peroxidases, including plant peroxidases derived from horseradish and soybeans. The key high-risk component of the research is the utilization of in situ extraction with the evaluation of the effects of the extraction solvents on enzymatic and catalytic activity. Both aqueous/organic mixtures and room temperature ionic liquids will be utilized for extraction and synthesis. Polymerization of 3-phenylphenol will be used to evaluate the activity of the enzymes. The broader impacts of the research include new avenues to environmentally and economically sustainable alternatives for biocatalysis synthesis. Publication will be through peer-reviewed journals. A strong K-12 outreach activity will be utilized. The project ensures strong participation of underrepresented groups. The combination of the extraction and synthesis environments would be a major advance in this area, and possible industrial applications would be very significant.

摘要 提案标题：SGER：用于酶合成的废弃生物材料 提案编号：CTS-0437544 首席研究员：Adrienne T. Cooper 机构：天普大学 该提案的目的是大幅扩展酶催化的实用性，以替代化学催化合成，以实现对环境无害的替代方案。 目前生物催化更广泛应用的障碍部分源于纯化酶（例如过氧化物酶）的高成本，以及对大量材料的需求。 有机溶剂的使用也很普遍。 然而，如果废弃生物材料可以用作酶的来源，则可以显着降低成本。 该项目将探索冻干过氧化物酶的潜在替代品，包括源自辣根和大豆的植物过氧化物酶。 该研究的关键高风险部分是利用原位萃取并评估萃取溶剂对酶和催化活性的影响。 水/有机混合物和室温离子液体都将用于萃取和合成。 3-苯基苯酚的聚合将用于评估酶的活性。 该研究的更广泛影响包括为生物催化合成提供环境和经济可持续替代品的新途径。 出版将通过同行评审的期刊进行。 将开展强有力的 K-12 外展活动。 该项目确保代表性不足的群体的大力参与。提取和合成环境的结合将是该领域的重大进步，并且可能的工业应用将非常重要。