SGER: Waste Biomaterials for Enzymatic Synthesis

摘要提案标题：SGER：用于酶合成的废弃生物材料提案编号：CTS-0437544主要研究者：Adrienne T.合作机构： 该提案的目的是大大扩大酶催化的实用性，用于化学催化合成的环境友好替代品。 目前生物催化更广泛应用的障碍部分是由于纯化酶如过氧化物酶的高成本，以及对大量材料的要求。 有机溶剂的使用也是普遍的。 然而，如果废弃的生物材料可以用作酶的来源，将实现显着的成本降低。 该项目将探索冻干过氧化物酶的潜在替代品，包括来自辣根和大豆的植物过氧化物酶。 该研究的关键高风险部分是利用原位提取，评估提取溶剂对酶和催化活性的影响。 水/有机混合物和室温离子液体都将用于萃取和合成。 3-苯基苯酚的聚合将用于评价酶的活性。 该研究的更广泛影响包括为生物催化合成提供环境和经济上可持续的替代品的新途径。 将通过同行评审期刊发表。 将利用强有力的K-12外联活动。 该项目确保代表性不足群体的积极参与。萃取和合成环境的结合将是这一领域的重大进展，可能的工业应用将是非常重要的。

AbstractProposal Title: SGER: Waste Biomaterials for Enzymatic Synthesis Proposal Number: CTS-0437544Principal Investigator: Adrienne T. CooperInstitution: Temple UniversityThe objective of this proposal is to substantially expand the utility of enzyme catalysis for environmentally benign alternatives to chemical catalysis syntheses. Present barriers to wider applications of biocatalysis stem in part to the high cost of purified enzymes, such as peroxidase, coupled with the requirements for large volumes of materials. Use of organic solvents also is prevalent. However, if waste biomaterials could be used as the source of the enzymes, dramatic cost reductions would be achieved. This project would explore promising potential replacements for lyophilized peroxidases, including plant peroxidases derived from horseradish and soybeans. The key high-risk component of the research is the utilization of in situ extraction with the evaluation of the effects of the extraction solvents on enzymatic and catalytic activity. Both aqueous/organic mixtures and room temperature ionic liquids will be utilized for extraction and synthesis. Polymerization of 3-phenylphenol will be used to evaluate the activity of the enzymes. The broader impacts of the research include new avenues to environmentally and economically sustainable alternatives for biocatalysis synthesis. Publication will be through peer-reviewed journals. A strong K-12 outreach activity will be utilized. The project ensures strong participation of underrepresented groups. The combination of the extraction and synthesis environments would be a major advance in this area, and possible industrial applications would be very significant.