PostDoctoral Research Fellowship in Polar Regions

极地博士后研究奖学金

• 批准号: 0443754
• 负责人: Bradley Buckley
• 金额: $ 12.92万
• 依托单位: Buckley Bradley A
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-11-01 至 2006-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0443754&HistoricalAwards=false
• 关键词: PostDoctoral; Research; Fellowship; Polar; Regions

This award is for a postdoctoral fellowship. The investigator will use molecular tools study the reasons why antarctic fish are unable to tolerate water warmer than about 6oC. These fish are extreme stenotherms, having evolved in sub-zero degree waters for somewhere between 5-15 million years. It is not known at this time whether the inability of these fish to tolerate increases in temperature is reflected in an inability to shift gene expression in response to heat stress. The proposed experiments will create the first cDNA (complementary DNA) microarray from Trematomus bernacchii, a common species in McMurdo Sound. The tissues of fish acclimatized over varying time periods to 4oC will be used to construct a complex, multi-tissue cDNA library of genes expressed during heat exposure. This library will be used to fabricate a cDNA microarray that will allow us to monitor the simultaneous expression of thousands of genes. RNA from control and heat shocked individuals will be used to create fluorescently tagged cDNA probes to be hybridized to the microarray, thereby generating gene expression profiles for thermally responsive genes. The primary hypothesis is that the ability to shift gene expression in response to elevated temperature will be absent in T. bernacchii and two related Antarctic species. The gene expression patterns from the Antarctic species will be compared to their temperate relatives from New Zealand. This project will increase our understanding of the genomic mechanisms related to cold-adaptation, stenothermality, and the response of polar fauna to environmental change.

这个奖项是为博士后奖学金。 研究人员将使用分子工具研究为什么南极鱼不能忍受温度超过6摄氏度的水。 这些鱼是极端的狭温动物，在零度以下的沃茨中进化了500 - 1500万年。目前尚不清楚这些鱼类无法耐受温度升高是否反映在无法改变基因表达以应对热应激。 拟议的实验将创建第一个cDNA（互补DNA）微阵列从Trematomus bernacchii，一个常见的物种在麦克默多声音。 在不同的时间段内适应4 ℃的鱼的组织将被用来构建一个复杂的，多组织的cDNA文库的基因在热暴露期间表达。这个文库将被用来制作cDNA微阵列，使我们能够监测数千个基因的同时表达。来自对照和热休克个体的RNA将用于产生荧光标记的cDNA探针以与微阵列杂交，从而产生热响应基因的基因表达谱。主要假设是T. bernacchii和两个相关的南极物种。来自南极物种的基因表达模式将与来自新西兰的温带亲属进行比较。该项目将增加我们对与冷适应、狭温性以及极地动物对环境变化的反应相关的基因组机制的理解。