The Cellular Stress Response in Cold-adapted Organisms: Building Novel Mechanistic Links between Heat Stress, Cell Cycle Arrest and Apoptosis in Antarctic Fishes.

适应寒冷的生物体的细胞应激反应：在南极鱼类的热应激、细胞周期停滞和细胞凋亡之间建立新的机制联系。

• 批准号: 0944743
• 负责人: Bradley Buckley
• 金额: $ 46.45万
• 依托单位: Portland State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-15 至 2013-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0944743&HistoricalAwards=false
• 关键词: Cellular; Stress; Response; Cold; adapted

The research will investigate a novel mechanism by which cold-adapted fishes of the Southern Ocean sense and respond to elevated temperatures. It is hypothesized that sub-lethal heat stress may induce cell cycle arrest and/or programmed cell death through apoptosis. The study will use genome-enabled technologies to examine the environmental control over gene expression in Antarctic species and will build direct mechanistic links between the expression of a specific signaling pathway gene and heat-induced changes in cells. Prior results support the hypothesis that heat stress results in cell cycle arrest and, in some cases, programmed cell death in Antarctic fishes. If so, this represents a novel, modified version of the well-conserved cellular stress response found in essentially all other species and suggests that warming ocean temperatures may have profound cellular and physiological impacts on these extremely stenothermal species. The P.I. conducts outreach activities with the Oregon Museum of Science and Industry, will be involved in developing a science curriculum for the Native American Youth and Family Center (NAYA) Early College Academy in Portland, and supports the educational and professional development of both undergraduate and graduate students at Portland State University.

这项研究将调查一种新的机制，通过这种机制，南大洋的冷适应鱼类能够感知和应对温度升高。据推测，亚致死热应激可能通过凋亡诱导细胞周期停滞和/或程序性细胞死亡。该研究将使用基因组技术来研究环境对南极物种基因表达的控制，并将在特定信号传导途径基因的表达与细胞中热诱导的变化之间建立直接的机制联系。先前的研究结果支持这一假设，即热应激导致细胞周期停滞，在某些情况下，程序性细胞死亡在南极鱼类。如果是这样的话，这代表了基本上所有其他物种中发现的保守细胞应激反应的一种新的修改版本，并表明变暖的海洋温度可能对这些极端狭窄的物种产生深远的细胞和生理影响。 私家侦探与俄勒冈州科学与工业博物馆一起开展外联活动，将参与为波特兰的美国土著青年和家庭中心（NAYA）早期学院学院制定科学课程，并支持波特兰州立大学本科生和研究生的教育和专业发展。