Supramolecular Formation, Complexation and Dynamics in Colloidal Polyelectrolyte-Surfactant Complexes

胶体聚电解质-表面活性剂复合物中的超分子形成、络合和动力学

• 批准号: 0454887
• 负责人: Benjamin Chu
• 金额: $ 34.5万
• 依托单位: SUNY at Stony Brook
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-03-15 至 2009-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0454887&HistoricalAwards=false
• 关键词: Supramolecular; Formation; Complexation; Dynamics; Colloidal

The emphasis in this project will be on soluble colloidal polyelectrolyte-surfactant complexes (CPSCs). The complex formation and supra-molecular assembly of colloidal particles with condensed and encapsulated polyelectrolytes, e.g., DNA fragments, require the introduction of more challenging designs on the nature of the surfactant and the surfactant molecular topology. Star tri-arm surfactants are proposed which consist of a hydrophilic block , a charged soluble block, and an additional biodegradable and flexible hydrophobic bloc (B), and which have the potential of being universal surfactants having micelle formation properties in both hydrophobic and hydrophilic environments. Kinetic processing is an essential element to manipulate the complex formation due to strong electrostatic interactions. Time-resolved observation of the binding process should play an important role in reaching a better understanding of the mechanism of C-PSC supra-molecular formation. The biodegradable hydrophobic block (B) in the surfactant will be designed to serve several useful functions. It can modify the hydrophobic surface of the PSC segments. The CPSC can thus be designed to be soluble in both the aqueous and hydrophobic environments. The presence of a duality of hydrophobic and hydrophilic chains on the PSC surface could increase the protection for the polyelectrolyte (e.g., DNA) in the CPSC. Furthermore the biodegradable block will be designed to destabilize the CPSCs for eventual release of the polyelectrolyte. Star tri-armed surfactants have synthesized . Fundamental studies will be carried out with emphasis on (i) the micellization of this new type of surfactants in selective solvents, (ii) their directed complex formation with polyelectrolytes in aqueous and hydrophobic environments, and (iii) the disassembly of CPSCs, in terms of (a) surfactant morphology and architecture, including chemical composition of the three blocks, total block length, and block ratio, (b) pH, (c) ionic strength, (d) the nature of counter ions, as well as solvent quality, concentration and temperature. Intellectual MeritThe proposed study deals with scientific investigations on the micro-phase behavior of polyelectrolyte condensation and encapsulation, using surfactants that can undergo hydrophobic and electrostatic interactions. The findings should provide new pathways on the design of better carriers for polyelectrolytes in general and for DNA fragments in particular. It could have an impact on the use of block copolymers as vehicles for gene and drug delivery, based on the coil-to-globule transition and the self-assembly of more complex block copolymers with oppositely charged polyelectrolyte. Its biological implication for in-vitro non-viral gene therapy applications could be especially fruitful from the polymer science viewpoint.Broader ImpactsThe proposed approach has potential for practical applications to drug and gene delivery, as well as tissue engineering. These are important topics that can take advantage of our knowledge in materials science for applications to biology and medicine. Furthermore, the project has unique and natural connections among the disciplines of polymer synthesis (chemistry), polymer processing (engineering) and characterization (physics), as well as polymer theory with specific timely objectives. The fertile grounds are appealing subject matters for the next generation of scientists and engineers, from high school to post-doctoral students.

本项目的重点是可溶性胶体聚电解质-表面活性剂复合物（CPSC）。胶体颗粒与凝聚和包封的聚电解质的复合物形成和超分子组装，例如，DNA片段，需要引入对表面活性剂的性质和表面活性剂分子拓扑结构更具挑战性的设计。提出了星星三臂表面活性剂，其由亲水性嵌段、带电荷的可溶性嵌段和另外的可生物降解且柔性的疏水性嵌段（B）组成，并且其具有成为在疏水和亲水环境中均具有胶束形成性质的通用表面活性剂的潜力。动力学处理是操纵由于强静电相互作用而形成的复合物的基本要素。结合过程的时间分辨观察应发挥重要作用，达到更好地理解C-PSC超分子形成的机制。表面活性剂中的可生物降解的疏水嵌段（B）将被设计成提供几种有用的功能。它可以修饰PSC片段的疏水表面。因此，CPSC可以被设计成可溶于水性和疏水性环境。PSC表面上疏水链和亲水链的二元性的存在可以增加对疏水链的保护（例如，在CPSC。此外，可生物降解的嵌段将被设计成使CPSC不稳定，以最终释放CPSC。合成了星星型三臂表面活性剂。基础研究将重点放在（i）这种新型表面活性剂在选择性溶剂中的胶束化，（ii）它们在水和疏水环境中与聚电解质的定向复合物形成，以及（iii）CPSC的分解，根据（a）表面活性剂形态和结构，包括三个嵌段的化学组成，总嵌段长度和嵌段比，（B）pH，（c）离子强度，（d）抗衡离子的性质，以及溶剂质量、浓度和温度。知识产权MeritThe拟议的研究涉及的微相行为的科学调查，使用的表面活性剂，可以进行疏水和静电相互作用的缩合和封装。这些发现将为设计更好的聚电解质载体，特别是DNA片段载体提供新的途径。它可能会对嵌段共聚物作为基因和药物递送载体的使用产生影响，这是基于线圈到小球的转变和具有相反电荷的自组装的更复杂的嵌段共聚物。从聚合物科学的角度来看，它对体外非病毒基因治疗应用的生物学意义可能特别富有成效。更广泛的影响所提出的方法具有在药物和基因递送以及组织工程方面实际应用的潜力。这些都是可以利用我们在材料科学方面的知识应用于生物学和医学的重要课题。此外，该项目在聚合物合成（化学），聚合物加工（工程）和表征（物理）以及具有特定时间目标的聚合物理论学科之间具有独特和自然的联系。对于下一代科学家和工程师来说，从高中到博士后，这片肥沃的土地都是吸引人的主题。