Formation of neuronal dendrite branches: the role of actin nucleators

神经元树突分支的形成：肌动蛋白成核剂的作用

• 批准号: 170387504
• 负责人: Professorin Dr. Gaia Tavosanis
• 金额: --
• 依托单位: Lehrstuhl für Entwicklungsbiologie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/170387504?language=en
• 关键词: Formation; neuronal; dendrite; branches; role

The establishment of correct neuronal dendrite morphology is essential for appropriate connectivity and information processing in the nervous system. In fact, defects in dendrite organization correlate with nervous system dysfunction. Nonetheless, the basic mechanisms underlying the formation of the complex and neuronal-type specific morphology of dendrites are still far from understood. Here, we propose to investigate which combinations of actin nucleators are involved in the formation of dendritic branches, a highly dynamic process. To this aim, we will use a well described group of neurons, the da neurons of the embryonic and larval peripheral nervous system of Drosophila. We will combine genetic and RNAi-mediated gene silencing with in vivo time-lapse studies and electron-microscopy, using a technique for improved actin preservation. Our goal is to examine all potential actin nucleators in Drosophila, establish their role in dendrite branching by loss- and gain-of-function experiments and to put this information into the context of the structural description provided by the electron-microscopy. We are confident that this combination of approaches will yield models of the function of actin nucleator complexes in vivo during the formation of dendrite branches.

正确的神经元树突形态的建立对于神经系统中适当的连接和信息处理是必不可少的。事实上，树突组织的缺陷与神经系统功能障碍有关。尽管如此，树突的复杂和神经元型特异性形态形成的基本机制仍远未被理解。在这里，我们建议调查的肌动蛋白成核剂的组合参与树突状分支的形成，一个高度动态的过程。为此，我们将使用一组已充分描述的神经元，即果蝇胚胎和幼虫周围神经系统的da神经元。我们将结合联合收割机遗传和RNAi介导的基因沉默与体内延时研究和电子显微镜，使用一种技术，以改善肌动蛋白的保存。我们的目标是研究果蝇中所有潜在的肌动蛋白成核剂，通过功能丧失和获得实验建立它们在树突分支中的作用，并将这些信息纳入电子显微镜提供的结构描述的背景下。我们相信，这种方法的组合将产生模型的功能，肌动蛋白成核剂复合物在体内形成树突分支。