A High-Throughput DNA Analyzer for Comparative Population Genomics

该奖项提供支持，使购买高通量DNA分析仪，热循环仪和机器人工作站被放置在一个核心的微量化学设施，它将使用和维护的设施工作人员。 这套设备现在是需要高通量DNA测序，微卫星分析和单核苷酸多态性调查的研究计划的支柱。研究和培训工作需要使用设备的研究者将按照服务计划收取固定费用。将这些设备放置在核心设施中，可以促进使用这些设备的不同研究小组之间的交流，从而分享想法、信息和技术创新。它还通过使设备提供给来自校园内不同学科和部门的大量其他用户，最大限度地利用设备。该设备将使PI及其同事能够通过增强片段分析（例如，微卫星DNA），更重要的是通过单核苷酸多态性调查。所要求的设备将被安置在耶鲁大学生物圈研究所-分子系统学和保护遗传实验室（YIBS-MSCG），该实验室培训学生应用于环境和生物体水平问题的分子技术。自成立以来，YIBS-MSCG通过研究轮换，正式的实验室课程，专门的研讨会和讲习班培训学生。这些设备将加强培训，使学生能够在适合其学业安排的时间内从大型数据集中获得遗传信息，并在培训预算范围内这样做。这也将使他们在科学生涯的早期接触到最先进的设备。该设备预计还将影响YIBS - MSCG与耶鲁皮博迪博物馆在与纽黑文公立学区的科学素养合作中的联合努力。通过这一方案，以来自低收入家庭的成绩不佳的少数民族学生为特点的中学成为教育推广的目标。两年前开始的合作伙伴关系计划将YIBS-MSCG实验室整合为一种提供额外学习和实践培训机会的手段。

This award provides support to enable the purchase of a high throughput DNA analyzer, thermocycler and robotic workstation to be placed in a core microchemistry facility where it will be used and maintained by facility staff. This set of equipment is now a mainstay of research programs requiring high throughput DNA sequencing, microsatellite analysis, and single-nuculeotide polymorphism surveys. Investigators whose research and training efforts require use of the equipment will be charged using a set fee for service schedule. Placement of such equipment in a core facility fosters communication among different research groups who use the equipment leading to the sharing of ideas, information, and technical innovations. It also maximizes the use of the equipment by making the equipment available to a large number of other users from different disciplines and departments across campus. The equipment will allow the PI and her colleagues to expand their capacity for population-level analysis via enhancement of fragment analysis (e.g., microsatellite DNA), and more importantly, via single nucleotide polymorphism survey. The requested equipment will be housed in the Yale Institute for Biospheric Studies - Molecular Systematics and Conservation Genetic laboratory (YIBS - MSCG) which trains students in molecular techniques applied to environmental and organismal level questions. Since its inception, the YIBS - MSCG has trained students via research rotations, formal laboratory courses, dedicated seminars, and workshops. The equipment will enhance training by allowing students to obtain genetic information from large data sets in a time frame that is appropriate to their academic schedules, and do so within the budget available for training. It will also expose them early in their scientific life to state of the art equipment. The equipment is also expected to impact joint efforts of YIBS - MSCG with the Yale Peabody Museum in a science literacy partnership with the public school district of New Haven. Via this program, middle schools characterized by underachieving minority students from low-income families are targeted for educational outreach. The partnership, begun two years ago, plans to integrate the YIBS-MSCG laboratory as a means for offering additional opportunities for learning and hands-on training.