DNA genetic analyzer for molecular ecology/biology applications

用于分子生态学/生物学应用的 DNA 遗传分析仪

• 批准号: RTI-2019-00600
• 负责人: Garant, Dany
• 金额: $ 10.89万
• 依托单位: Université de Sherbrooke
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=651521
• 关键词: DNA; genetic; analyzer; molecular; ecology

The objective of this proposal is to obtain a DNA genetic analyzer (AB 3500) that will represent the core of our molecular ecology/biology facility at Université de Sherbrooke. Sanger sequencing is a critically needed method to perform DNA fragment analysis and achieve our research objectives. Our research programs have substantially expanded in scope and productivity in recent years, resulting in a significant increase in the number of researchers needing to conduct genetic analyses. We currently have an AB 3130xl that critically needs to be replaced as it was acquired more than 10 years ago and it is reaching its end-of-life utility, which means increased repair costs for parts that are not covered by a warranty anymore. Also, the current equipment, which is discontinued since 2011, will no longer be supported by the manufacturer at the end of this year. It is thus vital to replace this equipment before a major breakdown, which would be catastrophic for our team research activities: the lack of daily, on-site access to such equipment will undermine our capacity to fulfil key steps in our respective research programs. The acquisition of the AB 3500 Series Genetic Analyzer, sold by Thermo Fisher, will solve this problem, as it is a top equipment in the field of capillary-electrophoresis-based analyses and as the company offers great maintenance and servicing plans. The infrastructure will support genotyping and sequencing molecular applications for at least eight Faculty members, their research teams as well as their collaborators. This equipment will satisfy our needs for analytical capacities for several years. The requested equipment will also allow our laboratories to maintain the recruitment and training of undergraduate research assistants, graduate students and postdoctoral fellows to work on our research programs. At least 50 HQP will be provided with hands-on training using this equipment over the next 5 years. Such training will improve their chances of obtaining a job of high quality in both private and public sectors. In summary, the requested DNA genetic analyzer is an essential equipment for our research group, which has been expanding over recent years. Our research is completely dependent on the acquisition of this equipment to remain productive and competitive at the international level.

该提案的目的是获得一个DNA遗传分析仪（AB 3500），它将代表我们在舍布鲁克大学的分子生态学/生物学设施的核心。桑格测序是进行DNA片段分析和实现我们的研究目标所急需的方法。近年来，我们的研究项目在范围和生产力方面都有了很大的扩展，导致需要进行遗传分析的研究人员数量显著增加。我们目前有一台AB 3130 xl，由于它是在10多年前购买的，并且即将达到使用寿命，因此急需更换，这意味着不再保修的部件的维修成本增加。此外，自2011年以来停产的现有设备将在今年年底不再得到制造商的支持。因此，在发生重大故障之前更换这些设备至关重要，这对我们的团队研究活动来说将是灾难性的：缺乏日常现场访问这些设备将削弱我们完成各自研究计划关键步骤的能力。收购由Thermo Fisher销售的AB 3500系列基因分析仪将解决这一问题，因为它是毛细管电泳分析领域的顶级设备，并且该公司提供了很好的维护和服务计划。该基础设施将支持至少八名教师，他们的研究团队以及他们的合作者的基因分型和测序分子应用。该设备将满足我们几年的分析能力需求。所要求的设备还将使我们的实验室能够继续招聘和培训本科生研究助理，研究生和博士后研究员，以从事我们的研究计划。在未来5年内，将向至少50名HQP提供使用该设备的实践培训。这种培训将增加他们在私营和公共部门获得高质量工作的机会。总之，所要求的DNA遗传分析仪是我们研究小组的必要设备，近年来一直在扩大。我们的研究完全依赖于购买这些设备，以保持在国际水平上的生产力和竞争力。