A family of novel G-protein coupled receptor-type proteins involved in cell wall integrity sensing

参与细胞壁完整性传感的新型 G 蛋白偶联受体型蛋白家族

• 批准号: 170526542
• 负责人: Professor Dr. Staffan Persson
• 金额: --
• 依托单位: Københavns Universitet
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2012-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/170526542?language=en
• 关键词: family; novel; protein; coupled; receptor

The plant cell wall is a complex network of carbohydrate-based polymers that controls overall plant morphogenesis. The constituents of the wall present essential components for several industries, including the food-, textile- and paper industries. In addition, the cell wall polymer cellulose, representing the most abundant biopolymer on earth, is estimated to become the main precursor for biofuels in the form of glucose-derived ethanol. Despite this, very little is known about how cellulose, and other cell wall polymers are produced, and how such production is controlled.Through a targeted reverse genetics screen we obtained several candidate genes associated with primary wall cellulose production. One of the genes corresponds to a putative seven transmembrane (7TM) protein with no known biological function. Curiously, several members of this protein family appear to have a role in cell wall integrity-, and sugar sensing. Preliminary results suggest that at least one of the 7TMs is a G-protein coupled receptor (GPCR). We therefore believe that the status of the cell wall architecture may be monitored via a GPCR mediated signaling scheme. We propose to functionally characterize the 7TM protein family, and to identify additional components associated with this signaling framework. The proposed research should lead to major advances in understanding the communication between the cell wall and the cell’s interior, and may result in the ability to manipulate the production of cell wall polymers for various industrial purposes.

植物细胞壁是一个复杂的网络碳水化合物为基础的聚合物，控制整个植物形态建成。该墙的组成部分是几个行业的基本组成部分，包括食品，纺织和造纸行业。此外，代表地球上最丰富的生物聚合物的细胞壁聚合物纤维素估计将成为葡萄糖衍生乙醇形式的生物燃料的主要前体。尽管如此，很少有人知道纤维素和其他细胞壁聚合物是如何产生的，以及这种生产是如何控制的。通过有针对性的反向遗传学筛选，我们获得了几个与初生壁纤维素生产相关的候选基因。其中一个基因对应于一个假定的七跨膜（7TM）蛋白，没有已知的生物学功能。奇怪的是，这个蛋白质家族的几个成员似乎在细胞壁完整性和糖传感中发挥作用。初步结果表明，至少有一个7TM是G蛋白偶联受体（GPCR）。因此，我们认为，细胞壁结构的状态可以通过GPCR介导的信号转导方案进行监测。我们建议在功能上表征7TM蛋白家族，并确定与此信号框架相关的其他组件。拟议的研究将导致在理解细胞壁和细胞内部之间的通信方面取得重大进展，并可能导致能够操纵用于各种工业目的的细胞壁聚合物的生产。