Multimodal Qdot Based Nanoprobe for Real Time Noninvasive Bioimaging

基于多模态 Qdot 的纳米探针，用于实时无创生物成像

• 批准号: 0506560
• 负责人: Brij Moudgil
• 金额: --
• 依托单位: University of Florida
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0506560&HistoricalAwards=false
• 关键词: Multimodal; Qdot; Based; Nanoprobe; Real

This proposal was received in response to Nanoscale Science and Engineering initiative, NSF 04-043, category NIRT. In a number of tissues, and particularly muscle and liver cells, stem cells are known to be recruited from their respective niches to repopulate or fuse with existent cells. However, the exact cellular signal and conditions that favor cell targeting and fusion are currently not well understood. This high risk proposal is to develop multimodal Qdot-based nanoprobes that are optically active to track cell delivery and are further acted upon when a labeled stem cell fuses with tissue expressing beta-galactose, to generate MR contrast. The nanoprobe will be engineered to both be excited by and emit in the near infrared, avoiding cell damage caused by higher frequency radiation and providing good transmission through bodily tissues, and to give high contrast during near infrared and magnetic resonance imaging, avoid the leaching of toxic metal ions, and provide flexibility of functionalization. Multimodal Qdots will offer the possibility of a revolutionary, ultra-sensitive (at the level of a single cell) bioimaging capability which will help to unravel the mystery of stem cell homing mechanisms and develop a strong foundation for understanding gene expression mechanisms.The proposed work will be linked to undergraduate programs, such as the Summer School in Particle Science and Research Experiences for Undergraduates programs, which will provide advanced seminars for experts and graduate students and research experiences for undergraduate students, respectively, based on the proposed work. A monthly seminar series will be started that will be open to the broad community, including professionals.

该提案是为了响应纳米科学与工程倡议、NSF 04-043、NIRT 类别而收到的。 在许多组织中，特别是肌肉和肝细胞中，已知干细胞会从各自的生态位中招募来重新填充或与现有细胞融合。 然而，目前尚不清楚有利于细胞靶向和融合的确切细胞信号和条件。这项高风险的提议是开发基于多模态 Qdot 的纳米探针，该探针具有光学活性，可跟踪细胞传递，并在标记的干细胞与表达 β-半乳糖的组织融合时进一步起作用，以产生 MR 对比。纳米探针将被设计为在近红外激发和发射，避免高频辐射引起的细胞损伤，并在身体组织中提供良好的传输，并在近红外和磁共振成像过程中提供高对比度，避免有毒金属离子的浸出，并提供功能化的灵活性。 多模态 Qdot 将提供革命性的、超灵敏（单细胞水平）生物成像能力的可能性，这将有助于揭开干细胞归巢机制的神秘面纱，并为理解基因表达机制奠定坚实的基础。拟议的工作将与本科生项目联系起来，例如粒子科学暑期学校和本科生研究经验项目，该项目将为专家和研究生提供高级研讨会和研究 根据拟议的工作分别为本科生提供经验。 将启动每月一次的研讨会系列，向包括专业人士在内的广大社区开放。