NER: Novel Cell Culture Stylus for the Rapid Assessment of Functional Nano-Bio Interfaces

NER：用于快速评估功能性纳米生物界面的新型细胞培养笔

• 批准号: 0609311
• 负责人: Brij Moudgil
• 金额: --
• 依托单位: University of Florida
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-15 至 2007-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0609311&HistoricalAwards=false
• 关键词: NER; Novel; Cell; Culture; Stylus

0609311MoudgilThis project will determine if adhesive interactions can be used to predict and isolate potentially beneficial or deleterious nanostructures for specific biological activity. It would be the first attempt to directly measure interaction forces between living cells and engineered nanostructures. This new cellular probe force microscopy (CPFM) technique will attach tissue culture to a probe that can assess large regions in a single experiment. Preliminary results show that the CPFM technique is sensitive to directly probe cell receptor-ligand binding. The project work plan was well presented with good justifications for each of the main experimental components. These justifications for experimental simplicity do eliminate more environmentally relevant research, however, the aim of the project is to demonstrate the feasibility of the tool which can then be applied to many different fields.

这个项目将确定粘合剂相互作用是否可以用来预测和分离潜在的有益或有害的特定生物活性的纳米结构。这将是首次尝试直接测量活细胞和工程纳米结构之间的相互作用力。这种新的细胞探针力显微镜（CPFM）技术将组织培养附着在探针上，可以在一次实验中评估大区域。初步结果表明，CPFM技术对直接探测细胞受体-配体结合具有敏感性。项目工作计划很好地提出了每个主要实验组成部分的充分理由。这些证明实验简单的理由确实排除了更多与环境有关的研究，然而，该项目的目的是证明该工具的可行性，然后可以将其应用于许多不同的领域。