SBIR Phase I: Production and Separation of Galacto-Oligosaccharides from Lactose for Prebiotic Food Applications

SBIR 第一阶段：从乳糖中生产和分离低聚半乳糖，用于益生元食品应用

• 批准号: 0512665
• 负责人: I-Ching Tang
• 金额: --
• 依托单位: Bioprocessing Innovative Company, Inc.
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2006-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0512665&HistoricalAwards=false
• 关键词: SBIR; Phase; Production; Separation; Galacto

This Small Business Innovation Research (SBIR) Phase I project will develop a novel immobilized enzyme process to produce galactooligosaccharides (GOS) from whey lactose for probiotic food applications. The proposed GOS production process involves two immobilized enzyme reactors and product separation by nanofiltration and adsorption chromatography. Two different alpha-galactosidase enzymes with different GOS formation characteristics will be used to optimize GOS production and yield from lactose. The product stream from the second reactor will be sent to a nanofiltration (NF) separation unit, where GOS, lactose, galactose, and glucose could be separated to yield a product with a higher GOS composition. The process for adsorption with activated carbon and ion exchange liquid chromatography will be developed to further purify GOS. The commercial application of this project will be in the emerging probiotic and neutraceutical food market for use by both animals and humans. This market is estimated to be in excess of 2 billion dollars per year. The present use of GOS in foods is limited by the high production costs. These costs are attributed mainly to the high enzyme cost and low oligosaccharide yields (less than 30% w/w). The proposed technology is expected to reduce production costs by at least 50% due by improving reactor productivity and enzyme life. Further, the ability to use cheap whey precursor products (current price range : $ 0.12 - $ 0.40 per lb) to value added GOS product (curent price : $ 4 - $ 5 per lb) will be of great benefit to the diary industry.

这个小企业创新研究（SBIR）一期项目将开发一种新的固定化酶工艺，从乳清乳糖中生产低聚半乳糖（GOS），用于益生菌食品。所提出的GOS生产工艺包括两个固定化酶反应器，并通过纳滤和吸附色谱分离产物。两种具有不同GOS形成特性的α -半乳糖苷酶将用于优化乳糖中GOS的生产和产量。来自第二个反应器的产品流将被送到纳滤（NF）分离装置，在那里可以分离GOS，乳糖，半乳糖和葡萄糖，以产生具有较高GOS成分的产品。研究了活性炭吸附法和离子交换液相色谱法对GOS的进一步提纯。该项目的商业应用将在新兴的益生菌和中性食品市场，供动物和人类使用。据估计，这个市场每年超过20亿美元。目前GOS在食品中的使用受到高生产成本的限制。这些成本主要归因于高酶成本和低寡糖产量（低于30% w/w）。该技术有望通过提高反应器生产率和酶的寿命，将生产成本降低至少50%。此外，将便宜的乳清前体产品（目前价格范围：每磅0.12美元至0.40美元）用于增值GOS产品（目前价格：每磅4美元至5美元）的能力将对乳制品行业大有裨益。