STTR Phase II: Engineering Clostridial Fermentation for Biobutanol Production

STTR 第二阶段：用于生物丁醇生产的梭菌发酵工程

• 批准号: 1026648
• 负责人: I-Ching Tang
• 金额: $ 50万
• 依托单位: Bioprocessing Innovative Company, Inc.
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-15 至 2013-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1026648&HistoricalAwards=false
• 关键词: STTR; Phase; II; Engineering; Clostridial

This STTR Phase II project will develop novel engineered Clostridia strains for fermentation and economically produce butanol as a biofuel from sugars derived from starchy and lignocellulosic biomass. The conventional acetone-butanol-ethanol (ABE)fermentation has low butanol yield (25%), butanol concentration (16 g/L), and reactor productivity (0.5 g/L?h) due to a strong butanol inhibition, and the fermentation process is difficult to improve due to the complicated metabolic pathways and gene regulation involved in the production microorganisms, mainly Clostridium acetobutylicum.The broader impact/commercial potential of the project is to produce butanol as a biofuel from sugars derived from starchy and lignocellulosic biomass. Biobutanol has great value as an alternative transportation fuel. There is a huge potential commercial and societal impact in improving yields and reducing costs of butanol production. The research and other activity proposed could lead directly to a marketable product and process and leads to several enabling technologies, including better manipulation of C. tyrobutylicum, further demonstration of strain improvements using the FBB, and others.

STTR 二期项目将开发用于发酵的新型工程梭菌菌株，并利用源自淀粉和木质纤维素生物质的糖经济地生产丁醇作为生物燃料。 传统的丙酮-丁醇-乙醇(ABE)发酵由于丁醇抑制作用强，导致丁醇收率(25%)、丁醇浓度(16 g/L)和反应器生产率(0.5 g/L·h)较低，而且由于生产微生物(主要是梭状芽胞杆菌)涉及复杂的代谢途径和基因调控，发酵过程难以改进。 该项目更广泛的影响/商业潜力是利用源自淀粉和木质纤维素生物质的糖生产丁醇作为生物燃料。 生物丁醇作为替代运输燃料具有巨大的价值。 提高丁醇生产的产量和降低成本具有巨大的潜在商业和社会影响。拟议的研究和其他活动可以直接产生可销售的产品和工艺，并带来多种使能技术，包括更好地操纵酪丁酸梭菌、使用 FBB 进一步证明菌株改进等。