STTR Phase II: Engineering Clostridial Fermentation for Biobutanol Production

这一STTR第二阶段项目将开发用于发酵的新型工程梭状芽胞杆菌菌株，并从淀粉和木质纤维生物质提取的糖中经济地生产丁醇作为生物燃料。传统的丙酮-丁醇-乙醇(ABE)发酵丁醇产率(25%)、丁醇浓度(16g/L)和反应器生产率(0.5g/L？h)都很低，丁醇抑制作用很强，发酵过程很难改进，因为生产微生物主要是乙酰丁酸梭菌，涉及复杂的代谢途径和基因调控。该项目更广泛的影响/商业潜力是利用淀粉和木质纤维生物质糖制取丁醇作为生物燃料。生物丁醇作为一种替代交通燃料具有很大的价值。丁醇生产在提高产量和降低成本方面具有巨大的潜在商业和社会影响。拟议的研究和其他活动可能直接导致适销对路的产品和过程，并导致几项使能技术，包括更好地操纵酪丁酸杆菌，使用FBB进一步展示菌株改良，以及其他。

This STTR Phase II project will develop novel engineered Clostridia strains for fermentation and economically produce butanol as a biofuel from sugars derived from starchy and lignocellulosic biomass. The conventional acetone-butanol-ethanol (ABE)fermentation has low butanol yield (25%), butanol concentration (16 g/L), and reactor productivity (0.5 g/L?h) due to a strong butanol inhibition, and the fermentation process is difficult to improve due to the complicated metabolic pathways and gene regulation involved in the production microorganisms, mainly Clostridium acetobutylicum.The broader impact/commercial potential of the project is to produce butanol as a biofuel from sugars derived from starchy and lignocellulosic biomass. Biobutanol has great value as an alternative transportation fuel. There is a huge potential commercial and societal impact in improving yields and reducing costs of butanol production. The research and other activity proposed could lead directly to a marketable product and process and leads to several enabling technologies, including better manipulation of C. tyrobutylicum, further demonstration of strain improvements using the FBB, and others.