SGER-Fluidic Sensors: Integrating Microfluidics with Biological Assays

SGER-流体传感器：将微流体与生物测定相结合

• 批准号: 0515711
• 负责人: Hugh Fan
• 金额: $ 7万
• 依托单位: University of Florida
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-08-15 至 2006-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0515711&HistoricalAwards=false
• 关键词: SGER; Fluidic; Sensors; Integrating; Microfluidics

Professor Hugh Fan of the University of Florida is supported with a Small Grant for Exploratory Research (SGER) from the Analytical and Surface Chemistry Program in the Division of Chemistry to study hydrogen gas sensing by enzyme-catalyzed electrochemical detection in a microfluidic device. The hydrogenase enzyme to be used in the sensor is obtained from microorganisms that consume hydrogen as their energy source. The electrons released during the enzymatic oxidation of hydrogen will be accepted by benzyl viologen, and the product of the redox reaction will be detected by chronoamperometry. Enzyme stability, selectivity, and sensitivity will be investigated.The idea is to develop a simple microfluidic hydrogen sensor that is portable and can quickly respond to hydrogen gas leaks. If successful, it will greatly aid the safe deployment of hydrogen-based energy sources as an alternate for fossil fuel.

佛罗里达大学的Hugh Fan教授获得了化学系分析和表面化学项目的探索性研究小额赠款（SGER）的支持，以研究通过微流体装置中的酶催化电化学检测进行氢气传感。 传感器中使用的氢化酶从消耗氢作为其能量源的微生物获得。 在氢的酶促氧化过程中释放的电子将被苄基紫精接受，并且氧化还原反应的产物将通过计时电流法检测。 本研究将探讨酵素的稳定性、选择性与灵敏度，并开发一种简单的微流控氢感测器，可携带且能快速回应氢气的泄漏。 如果成功，它将大大有助于氢基能源作为化石燃料替代品的安全部署。