RUI: Analysis of AXR4's Function in Membrane Trafficking in Arabidopsis

RUI：AXR4 在拟南芥膜运输中的功能分析

• 批准号: 0517027
• 负责人: Lawrence Hobbie
• 金额: --
• 依托单位: Adelphi University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-15 至 2009-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0517027&HistoricalAwards=false
• 关键词: RUI; Analysis; AXR4; Function; Membrane

Many essential functions of plant cells, such as taking up and exporting nutrients and hormones, are carried out by transport proteins embedded in the cell membrane. Proper activity of some of these transport proteins depends on their localization to one specific part of the cell membrane. The goal of this project is to increase our understanding of the mechanisms by which these membrane transport proteins are directed to the proper place in the cell. The study focuses on the AXR4 gene, which has been shown in the widely-studied plant Arabidopsis thaliana to be necessary for proper location of a protein that imports the hormone auxin. Genetic and molecular approaches will be used to identify genes and proteins that interact with AXR4 and act in the same pathway, which will reveal the cellular role of the AXR4 gene product. The project will enhance our knowledge of basic mechanisms of plant cell function, which may ultimately enable scientists to improve the growth of important crop plants. The project also will support the training in modern research techniques of undergraduates and master's students at a teaching-oriented institution.

植物细胞的许多基本功能，例如吸收和输出营养物质和激素，都是通过嵌入细胞膜的转运蛋白来实现的。其中一些转运蛋白的适当活性取决于它们对细胞膜某一特定部分的定位。该项目的目标是加深我们对这些膜转运蛋白被引导至细胞中适当位置的机制的理解。这项研究的重点是 AXR4 基因，该基因已在广泛研究的拟南芥植物中被证明对于正确定位导入激素生长素的蛋白质是必需的。遗传和分子方法将用于识别与 AXR4 相互作用并在同一途径中发挥作用的基因和蛋白质，这将揭示 AXR4 基因产物的细胞作用。该项目将增强我们对植物细胞功能基本机制的了解，最终可能使科学家能够改善重要农作物的生长。该项目还将支持教学型机构本科生和硕士生的现代研究技术培训。