Genetic Analysis of Methylotrophic Methanogenesis

甲基营养型产甲烷作用的遗传分析

• 批准号: 0517419
• 负责人: William Metcalf
• 金额: $ 45万
• 依托单位: University of Illinois at Urbana-Champaign
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0517419&HistoricalAwards=false
• 关键词: Genetic; Analysis; Methylotrophic; Methanogenesis

The long-term goal of the project is to expand our knowledge regarding the physiology, metabolism and evolutionary adaptation of Methanosarcina species. The primary emphasis of these studies will be the identification and characterization of genes that directly and indirectly effect the utilization of methanol as a growth substrate. These studies are expected to reveal novel aspects of the methylotrophic methanogenic pathway, and lend insight into the integration of methanogenesis into the global metabolism of the organism. Moreover, a clearer understanding of archaeal transcriptional control should result from examination of the highly regulated methylotrophic genes. Lastly, new genetic methods, which may be widely applicable, will be developed during the course of these studies. The proposed experiments will primarily utilize recently developed methods for genetic analysis of Methanosarcina species; however, physiological, biochemical and molecular approaches will complement the genetic approach. This strategy is expected to generate data that are both novel and complementary to the results of preceding studies. These studies will be broadly applicable to the field of methanogenesis, which is important to a number of human-related problems including the production of alternative fuels from biological materials, waste treatment, and global warming. This project will provide training opportunities for two graduate students and several undergraduate students.

该项目的长期目标是扩大我们对甲烷八叠球菌属物种的生理学，代谢和进化适应的了解。这些研究的主要重点将是鉴定和表征直接和间接影响甲醇作为生长底物利用的基因。这些研究有望揭示甲基营养型产甲烷途径的新方面，并深入了解甲烷合成到生物体的全球代谢中。此外，一个更清晰的了解古细菌的转录控制应导致高度调节的甲基营养基因的检查。最后，在这些研究过程中，将开发可能广泛适用的新遗传方法。拟议的实验将主要利用最近开发的甲烷八叠球菌属物种的遗传分析方法;然而，生理学，生物化学和分子方法将补充遗传方法。这一战略预计将产生新的和补充以前的研究结果的数据。这些研究将广泛适用于甲烷生成领域，这对许多与人类相关的问题非常重要，包括从生物材料生产替代燃料，废物处理和全球变暖。该项目将为两名研究生和几名本科生提供培训机会。