Acquisition of Field-emission Scanning Electron Microscopy at the Bigelow Laboratory for Ocean Sciences

在毕格罗海洋科学实验室获得场发射扫描电子显微镜

• 批准号: 0521230
• 负责人: Charles OKelly
• 金额: $ 36.1万
• 依托单位: Bigelow Laboratory for Ocean Sciences
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-08-01 至 2007-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0521230&HistoricalAwards=false
• 关键词: Acquisition; Field; emission; Scanning; Electron

A grant has been awarded to the Bigelow Laboratory for Ocean Sciences, under the direction of Dr. Charles O'Kelly, to support, in part, the acquisition of a field-emission scanning electron microscope with elemental analysis capability. Scanning electron microscopy (SEM) is an important tool to study the biodiversity of microbes, including protists (algae and protozoa), bacteria and viruses, in the marine environment. Both the morphology and the chemical composition of cell surface features, such as scales, cell wall components, cysts, mineral accretions) may provide characters that identify species. These features may help us discover how microbes interact with other organisms in the sea. They may also help us discover whether microparticles that contribute to ocean chemical fluxes, especially of carbon, have come from microbes, and if so from which ones. Often, the structures of interest are so small than most SEM instruments cannot provide clear images of them. Field-emission SEM has the resolving power to visualize these structure with the detail that we require, and can tell us with greater accuracy of what they are made.Educational activities include the development of a course on electron microscopy and its application to marine biological research, to be developed as part of Bigelow Laboratory's "Foundations of Marine Science" course series, and to be targeted particularly for Maine researchers and educators. Images from the SEM will also be incorporated into the "Phytopia" series of multimedia tools, intended for classroom use at Grade 7 and above and so used by numerous educators in the United States and throughout the world.Acquisition of the field-emission SEM will provide scientists at the Bigelow Laboratory with a greater range and depth of research opportunities, permitting expansion into areas previously out of reach for lack of appropriate instrumentation. The acquisition will promote greater use of Bigelow Laboratory facilities, especially the Provasoli-Guillard National Center for Culture of Marine Phytoplankton, by junior and senior visiting scientists. It will promote the development of educational programs and tools related to electron microscopy, through the Phytopia project series and other Bigelow Laboratory educational and outreach activities.

已向Charles O 'Kelly博士指导下的Bigelow海洋科学实验室提供了一笔赠款，以部分支持购置一台具有元素分析能力的场发射扫描电子显微镜。扫描电子显微镜（SEM）是研究海洋环境中原生生物（藻类和原生动物）、细菌和病毒等微生物生物多样性的重要工具。 细胞表面特征的形态学和化学组成，如鳞片、细胞壁成分、孢囊、矿物质增生）都可以提供识别物种的特征。 这些特征可能有助于我们发现微生物如何与海洋中的其他生物相互作用。 它们还可以帮助我们发现，造成海洋化学通量的微粒，特别是碳，是否来自微生物，如果是，来自哪些微生物。 通常，感兴趣的结构是如此之小，以至于大多数SEM仪器无法提供它们的清晰图像。 场致发射扫描电镜的分辨能力使这些结构具有我们所要求的细节，并能更准确地告诉我们它们是由什么组成的。教育活动包括发展一门关于电子显微镜及其在海洋生物研究中的应用的课程，这门课程将作为毕格罗实验室“海洋科学基础”系列课程的一部分，特别是缅因州的研究人员和教育工作者。 扫描电镜的图像也将被纳入“Phytopia”系列多媒体工具中，供7年级及以上的课堂使用，因此被美国和世界各地的许多教育工作者使用。获得场发射扫描电镜将为毕格罗实验室的科学家提供更广泛和更深入的研究机会，允许扩展到以前由于缺乏适当的仪器而无法达到的领域。 此次收购将促进初级和高级访问科学家更多地使用毕格罗实验室的设施，特别是普罗瓦索利-吉拉德国家海洋浮游植物培养中心。 它将通过Phytopia项目系列和毕格罗实验室的其他教育和推广活动，促进与电子显微镜相关的教育计划和工具的发展。