Post-translational protein arginylation in plants: localization, dynamics and function

植物中翻译后蛋白质精氨酰化：定位、动力学和功能

• 批准号: 172271801
• 负责人: Dr. Gabor Igloi
• 金额: --
• 依托单位: Institut für Biologie III
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/172271801?language=en
• 关键词: Post; translational; protein; arginylation; plants

We will investigate post-translational arginylation of plant proteins in the model organism Physcomitrella patens, a moss. The aim is to comprehend the role of protein arginylation, its impact on plant development and its influence on the function of selected proteins. We will characterize the changes that occur in response to an arginine-like allelochemical, canavanine. Using modern mass spectrometric tools of proteomics, combined with genetically altered plants lacking the arginylation capability, we will identify proteins that are targets for arginylation as well as factors that are involved in, and may control the modification process. The functional consequences of arginylation of selected proteins will be investigated in vitro.

我们将研究模式生物立碗藓（一种苔藓）中植物蛋白的翻译后精氨酸化。目的是了解蛋白质精氨酸化的作用、其对植物发育的影响及其对所选蛋白质功能的影响。我们将描述响应精氨酸样化感物质刀豆氨酸而发生的变化。利用蛋白质组学的现代质谱工具，结合缺乏精氨酸化能力的转基因植物，我们将鉴定出精氨酸化目标的蛋白质以及参与并可能控制修饰过程的因子。将在体外研究所选蛋白质精氨酸化的功能后果。