Post-translational protein arginylation in plants: localization, dynamics and function

我们将研究模式生物小Physcomitrella patens（一种苔藓）中植物蛋白的翻译后精氨酸化。目的是了解蛋白质精氨酸化的作用，其对植物发育的影响及其对选定蛋白质功能的影响。我们将描述对一种类似精氨酸的化感化学物质——菜豆氨酸的反应所发生的变化。利用蛋白质组学的现代质谱分析工具，结合缺乏精氨酸化能力的转基因植物，我们将确定精氨酸化的靶蛋白以及参与和可能控制修饰过程的因素。精氨酸化对选定蛋白的功能影响将在体外进行研究。

We will investigate post-translational arginylation of plant proteins in the model organism Physcomitrella patens, a moss. The aim is to comprehend the role of protein arginylation, its impact on plant development and its influence on the function of selected proteins. We will characterize the changes that occur in response to an arginine-like allelochemical, canavanine. Using modern mass spectrometric tools of proteomics, combined with genetically altered plants lacking the arginylation capability, we will identify proteins that are targets for arginylation as well as factors that are involved in, and may control the modification process. The functional consequences of arginylation of selected proteins will be investigated in vitro.