Biophotonics: Novel Lanthanide Bioassays

Faris0523077该提案概述了一个基于弱荧光或发光稀土络合物的共振增强而开发功能化分子探针的项目。稀土络合物具有作为荧光指示剂的一些有吸引力的性质，但存在吸收截面和量子效率低的问题。在作为天线的金属颗粒或岛的存在下，稀土络合物的亮度可以增强。由于这种增强随距离的不同而不同，因此该增强可用作报告局部生物活性的功能化或激活策略。这项建议将重点放在低量子效率的稀土络合物上，这些络合物很少受到关注，但有可能具有最大的相对增强效应，因此作为报告剂的动态范围最大。

Faris0523077This proposal outlines a project to develop functionalized molecular probes based on resonance enhancement of weakly fluorescent or luminescent lanthanide chelates. Lanthanide chelates have several attractive properties for use as fluorescent indicators, but suffer from low absorption cross-sections and quantum efficiency. The brightness of lanthanide chelates can be enhanced in the presence of metal particles or islands that act as antennas. Since this enhancement varies with proximity, the enhancement can be use as a functionalizing, or activation strategy for reporting local biological activity. This proposal will focus on low quantum efficiency lanthanide chelates which have received little attention but stand to have the largest relative enhancement effect, and therefore the greatest dynamic range as a reporting agent.