MRI: Development of a Stimulated Scattering Microscope for Cell Imaging

MRI：开发用于细胞成像的受激散射显微镜

• 批准号: 1229808
• 负责人: Gregory Faris
• 金额: $ 38.54万
• 依托单位: SRI International
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-08-15 至 2016-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1229808&HistoricalAwards=false
• 关键词: MRI; Development; Stimulated; Scattering; Microscope

ABSTRACTThe objective of the proposed study is to develop a new type of nonlinear scattering microscopy based on stimulated Rayleigh/Brillouin scattering that will enable measurement of thermal and elastic properties of cells. A central part of this work is assembly of a novel laser system that will provide very rapid tuning and excellent noise properties. In this effort SRI will (1) assemble the laser system, (2) combine the laser system with its associated electronics, and (3) evaluate instrument performance and operation through imaging of cells. This work will bring a new type of nonlinear microscopy to biology and medical research that will complement existing nonlinear microscopy techniques, including multiphoton microscopy using fluorescence, second-harmonic and third harmonic generation, coherent anti-Stokes Raman (CARS) microscopy, and four-wave mixing. SRI has recently developed new advances in stimulated Rayleigh/Brillouin scattering that will enable rapid imaging of cells without causing damage to the cells. The microscope SRI proposes to develop will allow a new look at effects such as signal transduction through mechanical forces, cell motility, cell division, and other aspects involving changes in cellular viscoelastic properties.

摘要本研究的目的是开发一种新型的非线性散射显微镜的基础上受激瑞利/布里渊散射，这将使测量的热和弹性性能的细胞。这项工作的一个核心部分是组装一个新的激光系统，将提供非常快速的调谐和优良的噪声特性。在这项工作中，SRI将（1）组装激光系统，（2）将激光系统与其相关电子设备结合起来，（3）通过细胞成像评估仪器性能和操作。联合收割机。这项工作将为生物学和医学研究带来一种新型的非线性显微镜，这将补充现有的非线性显微镜技术，包括使用荧光、二次谐波和三次谐波产生的多光子显微镜、相干反斯托克斯拉曼（汽车）显微镜和四波混合。SRI最近在受激瑞利/布里渊散射方面取得了新的进展，这将使细胞快速成像而不会对细胞造成损害。显微镜SRI建议开发将允许一个新的视角，如通过机械力，细胞运动，细胞分裂和其他方面的信号转导的影响，涉及细胞粘弹性的变化。