DDI--Molecular systematics of red colobus monkeys (Procolobus: Primates, Cercopithecidae)

DDI--红疣猴的分子系统学（原疣猴：灵长类动物，猴科）

• 批准号: 0524990
• 负责人: Eric Delson
• 金额: --
• 依托单位: CUNY Graduate School University Center
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-08-01 至 2006-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0524990&HistoricalAwards=false
• 关键词: DDI; Molecular; systematics; red; colobus

Red colobus monkeys (subfamily Colobinae, genus Procolobus, subgenus Piliocolobus) are distributed across equatorial Africa in over 14 different forms and display a complex pattern of variation. They currently rank among the most endangered primates in Africa, and their classification remains one of the longest-standing problems in African primate taxonomy. Using molecular techniques, this research will build a phylogeny and classification for red colobus monkeys, representing the most comprehensive molecular study of African leaf monkeys to date. Specifically, this research has the following objectives; 1) establish relationships among red colobus populations, 2) develop a taxonomy that accurately reflects the diversity within the group, 3) provide distributional information on red colobus populations, and 4) further our knowledge concerning colobine evolutionary history. Fecal, tissue from museum collections, and hair samples from numerous red colobus populations have been collected and will be subjected to DNA extraction and sequencing; GPS data collected in the field will be used to map geographical distributions; and molecular phylogenetic methods will be employed to estimate nuclear and mitochondrial DNA gene trees, which will be used to address questions concerning colobine systematics and the timing of vicariance events within the group. The results will lead to a better understanding of the behavioral ecology, morphology, and evolutionary radiation of the leaf monkeys, and allow the testing of hypotheses concerning African primate biogeography. Among the most important broader impacts of this project are the conservation implications. When there is no universal agreement on species-level classification, identification of endangered species becomes problematic, making the designation of conservation priorities difficult. Because one of the goals of conservation is to preserve genetic diversity, and taxonomy is meant to reflect phylogenetic relationships and genetic distinctiveness, well-resolved phylogenies are essential for effective conservation. Since red colobus relationships are currently poorly resolved, this study has implications for effective species-level conservation, biodiversity analysis, and designation of conservation priorities. Also, while in the field collecting samples, the student Co-PI collected distributional information for endangered red colobus populations, and hired and trained local field assistants. This Co-PI will also be instrumental in the tutelage of undergraduates in a molecular anthropology laboratory. The results of this research will be published in scholarly journals and presented at academic conferences, and sequences will be submitted to the publicly accessible GenBank database hosted by the National Center for Biotechnology Information (NCBI). Furthermore, this research will foster the professional development of a doctoral student.

赤眼猴(Colobinae亚科，Procolbus属，Piliocolobus亚属)以14种不同的形式分布在赤道非洲各地，并显示出复杂的变异模式。它们目前是非洲最濒危的灵长类动物之一，它们的分类仍然是非洲灵长类分类中存在时间最长的问题之一。利用分子技术，这项研究将建立红鳞猴的系统发育和分类，代表了迄今为止对非洲叶猴最全面的分子研究。具体地说，本研究有以下目标：1)建立种群间的亲缘关系；2)建立一个准确反映种群内多样性的分类学；3)提供种群的分布信息；4)加深我们对孔雀进化史的了解。来自博物馆藏品的粪便、博物馆藏品的组织和来自许多红根瘤虫种群的毛发样本已经收集，并将进行DNA提取和测序；野外收集的GPS数据将用于绘制地理分布图；将利用分子系统学方法估计核和线粒体DNA基因树，这将用于解决关于椰子虫系统学和种群内发生变化事件的时间的问题。这些结果将有助于更好地了解叶猴的行为生态学、形态和进化辐射，并允许检验有关非洲灵长类生物地理学的假说。该项目最重要的更广泛的影响是对保护的影响。如果在物种一级的分类上没有普遍的共识，识别濒危物种就成了问题，使得指定保护优先事项变得困难。因为保护的目标之一是保护遗传多样性，而分类学是为了反映系统发育关系和遗传独特性，所以良好的系统发育对于有效的保护至关重要。由于红结核的关系目前没有得到很好的解决，这项研究对有效的物种水平保护、生物多样性分析和指定保护优先事项具有重要意义。此外，在野外采集样本时，这名学生Co-Pi收集了濒危红根瘤菌种群的分布信息，并聘请和培训了当地的野外助理。这个合作项目还将在分子人类学实验室的本科生辅导中发挥重要作用。这项研究的结果将发表在学术期刊上并在学术会议上展示，序列将提交给由国家生物技术信息中心(NCBI)主持的公开可访问的GenBank数据库。此外，本研究还将促进博士生的专业发展。