Functional Sea Ice Ecology: Molecular Biodiversity, Metatranscriptomics and Metagenomics

功能性海冰生态学：分子生物多样性、宏转录组学和宏基因组学

• 批准号: 172485751
• 负责人: Professor Dr. Stephan Frickenhaus
• 金额: --
• 依托单位: Zentrum für Industriemathematik (ZeTeM)
• 依托单位国家: 德国
• 项目类别: Infrastructure Priority Programmes
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/172485751?language=en
• 关键词: Functional; Sea; Ice; Ecology; Molecular

During an earlier DFG project within the “Antarktisschwerpunktprogramm” (VA 105/13-1&2) we have established a seminal molecular biodiversity and metatranscriptomic dataset from selected eukaryotic polar sea ice communities. Phylogenetic analyses of both rDNA sequences and cDNA sequence fragments congruently revealed diatoms, haptophytes, and alveolates as the dominant taxa. For some of these, full genome sequencing projects are underway or just finished. Recently we obtained funding from Roche to sequence up to 3x109 basepairs of total DNA from sea ice communities. The aim of this proposal is to integrate the information available from genome sequences of sea ice organisms with environmental sequences including SSU rDNA, metatranscriptomic and metagenomic data. In particular, we intend to 1) assess “total” and “active” eukaryotic biodiversity, 2) identify genomic features differentiating sea ice organisms from other marine protists, and to 3) compare the active transcriptomes to the corresponding metagenomes and thus identify groups of genes / gene functions which are potentially involved in enabling these organisms to survive in sea ice. This will provide novel insights into genome composition, its variation and expression under extreme environmental conditions in eukaryotic sea ice microorganisms, and allow conclusions on the adaptive potential of sea ice communities, an ecosystem particularly endangered by expected global warming.

在早期的DFG项目“Antarktisschwerpunktprogramm”（VA 105/13-1&2），我们已经建立了一个开创性的分子生物多样性和元转录组数据集，从选定的真核极地海冰社区。rDNA序列和cDNA序列片段的系统发育分析一致显示硅藻，附着植物和肺泡作为优势类群。对于其中一些，全基因组测序项目正在进行或刚刚完成。最近，我们获得了罗氏公司的资助，对来自海冰群落的总DNA进行高达3x 109个碱基对的测序。该提案的目的是整合海冰生物基因组序列与环境序列，包括SSU rDNA，元转录组和宏基因组数据的信息。特别是，我们打算1）评估“总的”和“活跃的”真核生物多样性，2）确定区分海冰生物与其他海洋原生生物的基因组特征，3）比较活跃的转录组与相应的宏基因组，从而确定可能参与使这些生物在海冰中生存的基因/基因功能。这将为真核海冰微生物在极端环境条件下的基因组组成、变异和表达提供新的见解，并允许对海冰群落的适应潜力得出结论，海冰群落是一种特别受预期全球变暖威胁的生态系统。

项目成果

High-resolution SAR11 ecotype dynamics at the Bermuda Atlantic Time-series Study site by phylogenetic placement of pyrosequences

百慕大大西洋时间序列研究地点的高分辨率 SAR11 生态型动态（通过焦磷酸序列的系统发育位置）

• DOI: 10.1038/ismej.2013.32
• 发表时间: 2013
• 期刊: The ISME Journal
• 作者: Vergin KL;Beszteri B;Monier A;Thrash JC;Temperton B;Treusch AH;Kilpert F;Worden AZ;Giovannoni SJ
• 通讯作者: Giovannoni SJ

In situ expression of eukaryotic ice-binding proteins in microbial communities of Arctic and Antarctic sea ice

• DOI: 10.1038/ismej.2015.43
• 发表时间: 2015-11-01
• 期刊: ISME JOURNAL
• 影响因子: 11
• 作者: Uhlig, Christiane;Kilpert, Fabian;Beszteri, Bank
• 通讯作者: Beszteri, Bank

Regional variability in eukaryotic protist communities in the Amundsen Sea

阿蒙森海真核原生生物群落的区域变异

• DOI: 10.1017/s0954102013000229
• 发表时间: 2013
• 期刊: Antarctic Science
• 影响因子: 1.6
• 作者: Wolf C;Frickenhaus S;Kilias ES;Peeken I;Metfies K
• 通讯作者: Metfies K

The Marine Microbial Eukaryote Transcriptome Sequencing Project (MMETSP): illuminating the functional diversity of eukaryotic life in the oceans through transcriptome sequencing.

• DOI: 10.1371/journal.pbio.1001889
• 发表时间: 2014-06
• 期刊: PLoS biology
• 影响因子: 9.8
• 作者: Keeling PJ;Burki F;Wilcox HM;Allam B;Allen EE;Amaral-Zettler LA;Armbrust EV;Archibald JM;Bharti AK;Bell CJ;Beszteri B;Bidle KD;Cameron CT;Campbell L;Caron DA;Cattolico RA;Collier JL;Coyne K;Davy SK;Deschamps P;Dyhrman ST;Edvardsen B;Gates RD;Gobler CJ;Greenwood SJ;Guida SM;Jacobi JL;Jakobsen KS;James ER;Jenkins B;John U;Johnson MD;Juhl AR;Kamp A;Katz LA;Kiene R;Kudryavtsev A;Leander BS;Lin S;Lovejoy C;Lynn D;Marchetti A;McManus G;Nedelcu AM;Menden-Deuer S;Miceli C;Mock T;Montresor M;Moran MA;Murray S;Nadathur G;Nagai S;Ngam PB;Palenik B;Pawlowski J;Petroni G;Piganeau G;Posewitz MC;Rengefors K;Romano G;Rumpho ME;Rynearson T;Schilling KB;Schroeder DC;Simpson AG;Slamovits CH;Smith DR;Smith GJ;Smith SR;Sosik HM;Stief P;Theriot E;Twary SN;Umale PE;Vaulot D;Wawrik B;Wheeler GL;Wilson WH;Xu Y;Zingone A;Worden AZ
• 通讯作者: Worden AZ