A Fluorosensor Sediment Profile Imaging Incubation System (F-SPII) for Sediment Biogeochemistry and Diagenetic Process Studies

P.I.Aler，Robert(SUNY-SB)提案#：0527105项目标题：用于沉积物生物地球化学和成岩过程研究的荧光传感器沉积物剖面成像孵化系统(F-SPII)项目摘要表层沉积物中的成岩反应和物质输运对浅海环境中的生物地球化学循环起着重要的控制作用。PIS建议开发一种沉积物剖面成像相机和培养系统，以便与平面光致荧光传感器集成。该系统将允许对海底沉积物中的二维化学模式和溶质运移速率进行快速现场勘察和操纵过程研究。在早期REMOTS技术的基础上，荧光传感器沉积物剖面和孵化系统将穿透海床上层10-15厘米，允许一套特定于溶质的传感器膜在垂直剖面上与上覆水和下伏沉积物直接接触。采样区将包括生物地球化学最活跃的沉积矿床区域。除了可见光成像外，该仪器还将能够以高空间分辨率对面积约300平方厘米的垂直剖面进行荧光强度、比率激发-发射响应和荧光寿命成像。随着高功率LED技术的快速发展，光学系统设计将容易地结合新的激发源。F-SPII系统将被捆绑和设计，主要用于河口、港口和陆架环境，但可能会进行修改，以适应更深层次的部署。图像将在船上进行实时监控和自动处理。最初的应用将利用现有的O2、pH和PCO2传感器箔，但该仪器将用于进一步优化它们，并评估和优化正在开发的新型平面传感器(复合材料、硫化氢、钙++)。F-SPII还将被用来检查天然荧光成分的分布，例如表层沉积物(例如腐殖质)和可能的感光色素中DOC的亚类。可选择部署一个孵化室，允许捕获已知体积的上覆水，用于沉积物-水溶质通量测量和荧光示踪剂注入。所获得的一套测量结果应能史无前例地阐明早期成岩再矿化速率和复杂的反应模式，并量化生物扰动带中的溶质迁移过程。

P.I. Aller, Robert (SUNY-SB) Proposal #: 0527105PROJECT TITLE: A Fluorosensor Sediment Profile Imaging Incubation system (F-SPII) for sediment biogeochemistry and diagenetic process studiesProject SummaryDiagenetic reactions and material transport in surface sediments exert major controls on biogeochemical cycling in shallow marine environments. The PIs propose to develop a sediment profile imaging camera and incubation system for integration with planar optode fluorescence sensors. The system will allow rapid in situ reconnaissance and manipulative process studies of 2-dimensional chemical patterns and solute transport rates in bottom deposits. Building on earlier REMOTS technology, the fluorosensor sediment profiling and incubation system will penetrate the upper 10 - 15 cm of the seabed, allowing direct contact of a set of solute specific sensor foils with overlying water and underlying sediment in vertical section. The sampled zone will encompass the most biogeochemically active region of sedimentary deposits. In addition to visible imaging, the instrument will be capable of fluorescence intensity, ratiometric excitation - emission response, and fluorescence lifetime imaging of the vertical section over areas ~300 cm2 and at high spatial resolution. The optical system design will readily allow incorporation of new excitation sources as high power LED technology rapidly advances. The F-SPII system will be tethered and designed for use primarily in estuarine, harbor, and shelf environments, but could be modified for deeper deployments. Images will be monitored and automatically processed realtime on shipboard. Initial applications will utilize existing O2, pH, and pCO2 sensor foils but the instrument will be used to further optimize them, and to evaluate and optimize novel planar sensors under development (composites, H2S, Ca++). The F-SPII will also be utilized to examine the distribution of naturally fluorescent components, such as subclasses of DOC in surface sediment (e.g., humics) and possibly photopigments. An incubation chamber will optionally be deployed, allowing capture of a known volume of overlying water for sediment - water solute flux measurements and fluorescent tracer injection. The suite of measurements obtained should provide unprecedented elucidation of early diagenetic remineralization rates and complex reaction patterns, as well as quantification of solute transport processes in the bioturbated zone.