Mathematical Modeling and Computer Simulation for Gene Expression and Regulation

基因表达和调控的数学建模和计算机模拟

• 批准号: 0527114
• 负责人: Chichia Chiu
• 金额: $ 10万
• 依托单位: Michigan State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-12-01 至 2007-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0527114&HistoricalAwards=false
• 关键词: Mathematical; Modeling; Computer; Simulation; Gene

The general goal of this project is to establish research collaborations between mathematicians and experimental biologists at the Michigan State University and to train the graduate students in doing research in interdisciplinary fields. The proposed project is for the PI to work with Professor David N. Arnosti, a leading scientist in Molecular Genetics, at the Department of Biochemistry and Molecular Biology, Michigan State University. The initial area of focus would be mathematical modeling and computer simulation for gene expression and regulation. A key objective of contemporary gene regulatory studies is to understand the activity of the transcriptional apparatus at a quantitative level. The central goal of this project is to meld modeling approaches with quantitative determinations of transcriptional switch activity for a limited set of relatively well-characterized transcriptional regulators in the Drosophila blastoderm embryo. Unlike other current high level network approaches that treat gene switches as black boxes, this project is to study in details the cis regulatory rules that affect the efficacy of transcriptional repressors in the context of blocks of activator sites within enhancer. The modeling of transcriptional regulation will aid mechanistic understanding of transcriptional regulatory mechanisms, provide new insights on the evolution of gene regulatory elements and networks, and will supply tools for predicting the quantitative output of cis regulatory elements.This IGMS project is jointly supported by the MPS Office of Multidisciplinary Activities (OMA) and the Division of Mathematical Sciences (DMS).

这个项目的总体目标是在密歇根州立大学的数学家和实验生物学家之间建立研究合作关系，并培训研究生在跨学科领域进行研究。提议的项目是让PI与密歇根州立大学生物化学和分子生物学系的分子遗传学领军科学家大卫·N·阿诺斯蒂教授合作。最初的重点领域将是基因表达和调控的数学建模和计算机模拟。当代基因调控研究的一个关键目标是在定量水平上了解转录装置的活动。该项目的中心目标是将建模方法与果蝇胚芽中一组相对特征较好的转录调控因子的转录开关活性的定量测定相结合。与目前将基因开关视为黑匣子的其他高级网络方法不同，该项目将详细研究在增强子内激活位点块的背景下影响转录抑制物有效性的顺式调控规则。转录调控的建模将有助于从机制上理解转录调控机制，为基因调控元件和网络的进化提供新的见解，并将为预测顺式调控元件的数量输出提供工具。该IGMS项目由MPS多学科活动办公室(OMA)和数学科学司(DMS)联合支持。