Starter Grant: Whole Genome Profiling of the Numerically-Dominant Ecotype of the Marine Cyanobacterium, Prochlorococcus, Grown Under a Simulated Daily Light/Dark Cycle

入门资助：在模拟每日光/暗循环下生长的海洋蓝藻、原绿球藻的数字主导生态型的全基因组分析

• 批准号: 0527944
• 负责人: Erik Zinser
• 金额: $ 5万
• 依托单位: University of Tennessee Knoxville
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0527944&HistoricalAwards=false
• 关键词: Starter; Grant; Whole; Genome; Profiling

The cyanobacterium Prochlorococcus is believed to be the most abundant photosynthetic organism in the oceans, and as such contributes significantly to global carbon cycling. The change in solar energy availability over the course of the 24 hour day has profound implications for the physiology of Prochlorococcus. Photosynthesis occurs during the day, while at night they must survive on energy reserves. Prochlorococcus is also unusual in that its cell division cycle is linked to the light - dark cycle: they replicate their chromosome in the afternoon and divide in the early evening. Gene expression also varies over the course of a light - dark cycle, as recent whole-genome profiling of strain MED4 has demonstrated. Most genes peaked either at the end of the night or end of the day, to prepare the cell for photosynthesis (in the day), respiration (in the night), cell division (in the night), or other cellular activities. This project is aimed at characterizing the whole-genome expression profile of a second strain of Prochlorococcus, MIT 9312, grown under the same conditions used for MED4. Strain MIT 9312 is in a different evolutionary lineage than MED4 (i.e. different species), and they have dramatically different distributions in the oceans. Given the central importance of the light - dark cycle to Prochlorococcus photophysiology and cell division, the different ecological distributions observed for the two lineages could be due (in part) to differences in the way that their cellular processes are coordinated with the light - dark cycle. A major focus of this project will therefore be the comparison of the gene expression profiles of the two strains. This project will involve the training of undergraduate and graduate students, and the results of this work will be presented at national and international conferences.

原绿球藻被认为是海洋中最丰富的光合生物，因此对全球碳循环做出了重大贡献。 在一天24小时内太阳能可用性的变化对原绿球藻的生理学有着深远的影响。 光合作用发生在白天，而在晚上，它们必须依靠能量储备生存。 原绿球藻的不寻常之处还在于它的细胞分裂周期与明暗周期有关：它们在下午复制染色体，在傍晚分裂。 基因表达也随着光-暗循环的过程而变化，如最近对菌株MED 4的全基因组分析所证明的。 大多数基因在夜晚或白天结束时达到峰值，为细胞进行光合作用（白天）、呼吸作用（夜间）、细胞分裂（夜间）或其他细胞活动做准备。 该项目的目的是表征在与MED 4相同的条件下生长的第二株原绿球藻MIT 9312的全基因组表达谱。 菌株MIT 9312与MED 4处于不同的进化谱系（即不同的物种），并且它们在海洋中具有显著不同的分布。 鉴于光-暗循环对原绿球藻生理学和细胞分裂的重要性，观察到的两个谱系的不同生态分布可能（部分）是由于它们的细胞过程与光-暗循环协调的方式不同。 因此，该项目的一个主要重点是比较两种菌株的基因表达谱。 该项目将涉及对本科生和研究生的培训，并将在国家和国际会议上介绍这项工作的成果。