SST: Biosensor arrays based on DNA shuffling and chemometrics for measurement of chlorinated solvent mixtures

这个项目的目标是创造一种新型的生物传感器系统，能够同时测量多个化学上相似的分析物的浓度。这项研究的重点是测量地下水中的氯代乙烯混合物，但这里开发的方法在生物医学、生物加工和食品工业中具有重要的潜在应用。该生物传感器系统将基于光纤氧气传感器(氧气光电传感器)。含有催化耗氧反应的单加氧酶的细胞将被层叠在这个光极的尖端，形成一个光学生物传感器。同时监测类似分析物的方法是捆绑一组这样的生物传感器，每个传感器都有不同的突变单加氧酶，对氯化乙烯的敏感性也不同。分析束中生物传感器的不同反应将得出单个化学品的浓度，即使是在混合物中也是如此。

0529048ReardonThe goal of this project is to create a new type of biosensor system capable of simultaneously measuring the concentrations of multiple, chemically similar analytes. The research focuses on the measurement of mixtures of chlorinated ethenes in groundwater, but the approaches developed here have important potential applications in the biomedical, bioprocessing, and food industries. This biosensor system will be based on a fiber optic oxygen sensor (oxygen optode). Cells containing monooxygenase enzymes, which catalyze an oxygen-consuming reaction, will be layered on the tip of this optode, forming an optical biosensor. The approach for simultaneously monitoring similar analytes is to bundle a set of these biosensors, each with a different mutated monooxygenase and different sensitivity to the chlorinated ethenes. Analysis of the differing responses of the biosensors in the bundle will yield the concentrations of the individual chemicals, even when present in a mixture.