SBIR Phase I: A Proteomic-Based Biometric System for Near-Real Time Detection of Less-Than-Lethal Exposures to CBRN Materials

这个小企业创新研究第一阶段项目是开发一种基于蛋白质组学的生物识别系统，用于近实时检测CBRN材料的亚症状暴露。蛋白质表达和翻译后修饰数据，如蛋白质阵列、LC/MS和凝胶电泳（1-D或2-D）数据将用于鉴定蛋白质表达变化。蛋白质组学数据将与基因表达数据和代谢组学/代谢组学数据相结合，以确定参与细胞对外部损伤反应的途径中这些蛋白质变化的上游和下游的分子事件。 第一阶段的工作将集中在定义和开发生物信息学工具，该工具将用于识别或链接到识别改变的蛋白质表达的数据库，以帮助分析和解释蛋白质组学数据。这一软件工具将能够量化和解释各种生物模式概况中复杂和随时间变化的全球变化。 开发的生物识别系统将具有军事和民用应用。它可以被生物技术和制药公司用来识别新的生物标志物，被环保组织用来评估和监测低水平摄入有毒物质的影响，被医疗保健行业用来监测和评估饮食和健身活动的有效性。它还可以被军方用来检测和诊断有毒物质的暴露并做出部署决定，并被国土安全部用来监测和检测可疑的恐怖活动并实施应急响应计划。

This Small Business Innovative Research Phase I project is to develop a proteomic-based biometric system for near-real time detection of sub-symptomatic exposures of CBRN materials. Protein expression and post-translational modification data such as protein arrays, LC/MS and gel electrophoresis (1-D or 2-D) data will be used to identify protein expression changes. The proteomics data will be integrated with gene expression data and metabolomics/metabonomics data to identify the molecular events up- and down-stream of these protein changes in the pathways involved in the cellular response to the external insults. Phase I effort will be focused on defining and developing the bioinformatics tool that will be used to identify or link to databases that identify altered protein expression, to aid in the analysis and interpretation of proteomics data. This software tool will be capable of quantifying and interpreting complex and time-dependent global changes in various biological pattern profiles. The developed biometric system will have both military and civilian applications. It can be used by biotech and pharmaceutical companies to identify new biomarkers, by environmental conscious organizations to assess and monitor effects of low-level intakes of toxic materials, by the health care industry to monitor and assess the effectiveness of diet and fitness activities. It can also be used by the military to detect and diagnose exposure of toxic materials and make deployment decisions, and by the homeland security to monitor and detect suspicious terrorist activity and implement emergency response plans.