Proteomic analysis of acute phase proteins of sea cucumber,Apostichopus japonicus

海参急性期蛋白的蛋白质组学分析

• 批准号: 22580208
• 负责人: NAKAMURA Osamu
• 金额: $ 2.83万
• 依托单位: Kitasato University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22580208/
• 关键词: マナマコ; 体腔球; リポ多糖; リポテイコ酸; ペプチドグリカン; ザイモサン; プロテオーム解析; C-type レクチン; 生体防御; 水産学; ナマコ

To investigate acute-phase response proteins in a sea cucumberApostichopus japonicus, the coelomic fluids containing coelomocytes were obtained fromseveral specimens and divided into tubes. Bacterila- or fungi-derived substances suchas LPS and zymosan were added to those coelomic fluids. After incubation for 48h, thesupaernatant of coelomic fluids were subjected to 2-D electrophoresis and compared withthat of controls which were received the buffer only. As a result, many spots that werenot seen in the control were detected in the stimulated fluids. We obtained N-terminalamino acid sequences of several protein spots, and found some unidentified sequences aswell as C-type lectins reported formerly.

为了研究仿刺参（Apostichopus）的急性时相反应蛋白，从几个样品中获得含有体腔细胞的体腔液，并分成试管。将细菌或真菌衍生的物质（例如LPS和酵母聚糖）添加到这些体腔液中。孵育48小时后，将体腔液上清进行双向电泳，并与仅接受缓冲液的对照进行比较。结果，在刺激液中检测到了许多在对照组中看不到的斑点。我们获得了几个蛋白质点的N-末端氨基酸序列，并发现了一些未鉴定的序列以及以前报道的C-型凝集素。