Proteomic analysis of acute phase proteins of sea cucumber,Apostichopus japonicus

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To investigate acute-phase response proteins in a sea cucumberApostichopus japonicus, the coelomic fluids containing coelomocytes were obtained fromseveral specimens and divided into tubes. Bacterila- or fungi-derived substances suchas LPS and zymosan were added to those coelomic fluids. After incubation for 48h, thesupaernatant of coelomic fluids were subjected to 2-D electrophoresis and compared withthat of controls which were received the buffer only. As a result, many spots that werenot seen in the control were detected in the stimulated fluids. We obtained N-terminalamino acid sequences of several protein spots, and found some unidentified sequences aswell as C-type lectins reported formerly.