Nanotox: Biochemical, Molecular and Cellular Responses of Zebrafish Exposed to Metallic Nanoparticles

0540920 Barber该提案涉及金属纳米颗粒对斑马鱼毒性作用的生物学、组织学和分子学研究。鱼将暴露在各种浓度的铝、银、镍和二氧化钛纳米颗粒的水悬浮液中96小时。将在鳃、肝、肠和肾组织的形态学、生物化学和分子水平上评价毒性效应。主要目标之一是比较溶解态和纳米态金属以及不同尺寸和形状的纳米颗粒的毒性。假设是金属纳米颗粒将产生与水溶液中的金属不同的毒性作用。该提案将利用新到达的斑马鱼微阵列来表征不同处理水平下的基因表达，然后对15个最突出的基因进行定量PCR。还将测试体外模型系统作为水生物种中金属纳米颗粒毒性测定的替代方法。

0540920 Barber The proposal is concerned with biological, histological and molecular studies of toxic effects of metal nanoparticles on zebrafish. The fish will be exposed for 96 hours to aqueous suspensions of aluminum, silver, nickel, and titania nanoparticles of various concentrations. Toxicity effects will be evaluated at the morphological, biochemical and molecular levels in gills, liver, intestine, and kidney tissues. One of the main objectives is to compare toxicity of metals in the dissolved and nanoparticle state as well as the nanoparticles of different sizes and shape. The hypothesis is that the metal nanoparticles will produce toxic effects that are different from that of metals in aqueous solution. The proposal will take advantage of newly arriving zebrafish microarrays to characterize gene expression at different treatment levels, followed by quantitative PCR of the 15 most prominent genes. An in vitro model system will also be tested as an alternative for a toxicity assay of metal nanoparticles in aquatic species.