RIG: Metabolite Target Analysis using Microdevice Electrophoresis

RIG：使用微器件电泳进行代谢物目标分析

• 批准号: 0542336
• 负责人: Gloria Thomas-Fuller
• 金额: $ 17.48万
• 依托单位: Mississippi State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-06-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0542336&HistoricalAwards=false
• 关键词: RIG; Metabolite; Target; Analysis; using

This project will develop new microdevice (microfluidic) electrophoresis methods to improve the separation and detection of metabolites unique to certain cellular processes. Electrophoresis, a technique used to separate molecules based on differential migration rates in an electric field, remains one of the most powerful and widely used analytical tools in biology and in chemistry. The modern capillary format, microdevice electrophoresis, offers numerous advantages over the traditional slab gel, such as sensitive on-line detection, speed, cost, and improved dissipation of joule heating. Microfluidic methods to analyze estrogen metabolites will be created using two different strategies, free solution and hydrogel immobilization. Both approaches will utilize competitive immunoassays. The free solution microfluidic method will employ conventional competitive immunoassays whereas the hydrogel immobilization strategy will rely on the physical entrapment of antibodies specific for estrogen metabolites within acrylamide gel plugs. Antigen specific hydrogels will be evaluated for their ability to capture and concentrate estrogen metabolites at various concentrations. Limits of detection and non-specific binding will be assessed to directly compare the utility and diverse applications of the two methods. The broader impacts of this research are linked to its potential to:1. train numerous minority graduate and postdoctoral students in interdisciplinary research, 2. enhance current outreach activities designed to give economically and socially disadvantaged high school students hands-on research experience, 3. pioneer knowledge relating to microfluidic electrophoresis, and4. revolutionize the speed and resolution with which scientists can analyze macromolecules.

该项目将开发新的微器件（微流体）电泳方法，以改善某些细胞过程特有的代谢物的分离和检测。 电泳是一种用于基于电场中的差异迁移率分离分子的技术，仍然是生物学和化学中最强大和最广泛使用的分析工具之一。 现代毛细管格式，微器件电泳，提供了许多优势，传统的平板凝胶，如灵敏的在线检测，速度，成本，并改善焦耳热耗散。 微流体方法来分析雌激素代谢物将创建使用两种不同的策略，自由溶液和水凝胶固定。 这两种方法都将利用竞争性免疫测定。 游离溶液微流控方法将采用常规的竞争性免疫测定，而水凝胶固定化策略将依赖于丙烯酰胺凝胶塞内的雌激素代谢物特异性抗体的物理截留。 将评价抗原特异性水凝胶捕获和浓缩不同浓度雌激素代谢物的能力。 将评估检测限和非特异性结合，以直接比较两种方法的实用性和不同应用。这项研究的更广泛的影响与其潜力有关：1。培养大批从事跨学科研究的少数民族研究生和博士后。加强目前的外联活动， 学校学生动手研究经验，3。与微流控电泳相关的开拓性知识;革命性地提高了科学家分析大分子的速度和分辨率。