CAA: Rhizosphere Influence on Hydrocarbon Metabolizing Microorganisms

CAA：根际对碳氢化合物代谢微生物的影响

• 批准号: 0542309
• 负责人: Gregory Colores
• 金额: $ 17.48万
• 依托单位: Central Michigan University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-01 至 2009-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0542309&HistoricalAwards=false
• 关键词: CAA; Rhizosphere; Influence; Hydrocarbon; Metabolizing

Gregory M. Colores Proposal #0542309Rhizosphere influence on hydrocarbon metabolizing microorganismsThe goal of this Career Advancement Award is to use cultivation and non-cultivation based methods to characterize the microbial populations associated with plant rhizospheres in hydrocarbon-impacted soils. A series of plots have been established in polynuclear aromatic hydrocarbon (PAH) impacted soils that have been planted with species native to Michigan. Preliminary results with these plots indicate that individual plant species have different effects on the extent of hydrocarbon removal. In this research project, experiments will be conducted to evaluate the different influences that unique plant species have on the microbial communities inhabiting the rhizospheres. This will be performed by analyzing soil samples collected from different plots at various time intervals. Microbial communities will be characterized by 1) sequencing a gene that will enable the identification of microorganisms present (16S rRNA genes) 2) sequencing genes involved in PAH-degradation (PAH dioxygenases), and 3) comparing carbon utilization profiles of rhizosphere samples. It is anticipated that these experiments will reveal microbiological factors that enable some plants to accelerate the removal of PAHs from contaminated soils, whereas others hinder their removal. The broader impacts include developing an ecological framework for understanding how an applied technology like phytoremediation can be optimized. Some aspects of this project will also be integrated into a semester long cooperative laboratory experience for a microbial ecology and plant physiology class taught during the same semester. Further, this support will be used to increase research opportunities for underrepresented populations through local outreach and through additional, formal NSF channels (e.g. REU and RET supplements).

Gregory M.Colore提案#0542309根际对碳氢化合物代谢微生物的影响这项职业发展奖的目标是使用基于栽培和非栽培的方法来表征与碳氢化合物影响土壤中的植物根际相关的微生物种群。在受多环芳烃(PAH)影响的土壤中种植了密歇根州本土物种，已经建立了一系列的地块。这些小区的初步结果表明，不同的植物物种对碳氢化合物的去除程度有不同的影响。在这项研究项目中，将进行实验，以评估独特的植物物种对根际微生物群落的不同影响。这将通过分析在不同时间间隔从不同地块收集的土壤样本来进行。微生物群落的特征将通过1)对能够识别存在的微生物的基因(16S rRNA基因)进行测序，2)对参与多环芳烃降解的基因(多环芳烃双加氧酶)进行测序，以及3)比较根际样品的碳利用概况。预计这些实验将揭示微生物因素，这些因素使一些植物能够加速从受污染的土壤中去除多环芳烃，而其他植物则会阻碍它们的去除。更广泛的影响包括建立一个生态框架，以了解如何优化植物修复等应用技术。这个项目的一些方面也将被整合到同一学期教授的微生物生态学和植物生理学课程的长达一学期的合作实验室体验中。此外，这项支持将用于通过地方推广和通过其他正式的国家科学基金渠道(例如，REU和RET补充资料)，为代表性不足的人群增加研究机会。