The Role of AtbZIP1 in Sugar Regulatory Network

AtbZIP1 在糖调节网络中的作用

• 批准号: 0543751
• 负责人: Jyan-Chyun Jang
• 金额: $ 48万
• 依托单位: Ohio State University Research Foundation -DO NOT USE
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-02-01 至 2010-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0543751&HistoricalAwards=false
• 关键词: Role; AtbZIP1; Sugar; Regulatory; Network

Regulation of cell signaling can occur at many different levels. One such signaling mechanism is the interaction between DNA elements and DNA-binding transcription factors (TFs), which can act as a regulatory circuit to turn on or turn off gene expression. Despite the fact that at least 10% of all Arabidopsis genes are sugar responsive, very few regulatory circuits are known to be associated with sugar signaling. Using micro array analysis, this investigator has identified 184 glucose responsive TFs in Arabidopsis. Forty-five of these TFs appear to be glucose responsive without requiring new protein synthesis, i.e. the glucose response cannot be eliminated by the protein synthesis inhibitor cycloheximide (CHX). The hypothesis is that sugar-responsive TFs play key roles in sugar signaling and that CHX-insensitive TFs modulate CHX-sensitive TFs to create an inter-connected regulatory network. To test this hypothesis, the PI will use a CHX-insensitive and sugar-responsive bZIP transcription factor as a model. Reverse genetic analyses will be conducted to unravel the role of this bZIP in plants sugar responses. The downstream target genes of the bZIP will be identified by chromatin immunoprecipitation coupled Gene Chip analysis (ChIP-chip). These studies will also identify the upstream regulators of the bZIP using both computational and wet-lab analyses. This research is expected to shed light on the global regulatory roles of sugar on gene expression in plants. This project will also provide interdisciplinary training opportunities for undergraduates, graduates, and postdoctoral researchers in the area of molecular genetics, biochemistry, functional genomics, and bioinformatics. The accumulated micro array data and a large portion of the output from this proposal will be integrated into web-based resources available at public depositories.

细胞信号传导的调节可以在许多不同的水平上发生。 一种这样的信号传导机制是DNA元件和DNA结合转录因子（TF）之间的相互作用，其可以充当开启或关闭基因表达的调节回路。 尽管事实上，至少有10%的拟南芥基因是糖响应，很少有调控电路是已知的糖信号。 利用微阵列分析，本研究者在拟南芥中鉴定了184个葡萄糖响应性转录因子。 这些TF中的45种似乎是葡萄糖响应性的，而不需要新的蛋白质合成，即葡萄糖响应不能被蛋白质合成抑制剂放线菌酮（CHX）消除。 该假说是糖响应性转录因子在糖信号传导中起关键作用，而CHX不敏感性转录因子调节CHX敏感性转录因子以创建相互连接的调节网络。 为了检验这一假设，PI将使用CHX不敏感和糖响应性bZIP转录因子作为模型。 将进行反向遗传分析，以揭示这种bZIP在植物糖反应中的作用。 bZIP的下游靶基因将通过染色质免疫沉淀偶联基因芯片分析（ChIP-chip）进行鉴定。 这些研究还将使用计算和湿实验室分析来确定bZIP的上游调节器。 这项研究有望揭示糖对植物基因表达的全球调控作用。 该项目还将为本科生、研究生和博士后研究人员提供分子遗传学、生物化学、功能基因组学和生物信息学领域的跨学科培训机会。积累的微阵列数据和该提案的大部分产出将被纳入公共寄存处提供的网络资源。