CAREER: CHEMICAL METHODS FOR SELECTIVE PROTEOME ANALYSIS

职业：选择性蛋白质组分析的化学方法

• 批准号: 0547940
• 负责人: Gavin Reid
• 金额: $ 51万
• 依托单位: Michigan State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-02-01 至 2011-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0547940&HistoricalAwards=false
• 关键词: CAREER; CHEMICAL; METHODS; SELECTIVE; PROTEOME

Professor Gavin E. Reid of Michigan State University is supported by the Analytical and Surface Chemistry Program in the Division of Chemistry to develop a range of new derivatization strategies that will allow the incorporation of a "fixed charge" onto targeted amino acid residues within peptides, to enable selective identification and quantitation of specific groups of peptides from complex mixtures of protein digests. The project will contribute to the fundamental knowledge in gas phase charge-directed peptide fragmentation, and will add new reagents and analytical tools for protein identification by mass spectrometry. The industrial outreach component of this project is expected to positively impact graduate and undergraduate students involved in this project.The emergence of mass spectrometry as a major bioanalytical tool is the product of both innovations in instrumentation and new ionization chemistries that can be applied to complex biomolecules. This work will have significant impact in areas of analytical chemistry, biological chemistry, and biology.

密歇根州立大学的Gavin E. Reid教授在化学系分析和表面化学项目的支持下，开发了一系列新的衍生化策略，这些策略将允许将“固定电荷”结合到肽内的目标氨基酸残基上，从而能够从复杂的蛋白质消化混合物中选择性地鉴定和定量特定的肽群。该项目将为气相电荷定向肽片段的基础知识做出贡献，并将为质谱鉴定蛋白质添加新的试剂和分析工具。本项目的产业拓展部分有望对参与本项目的研究生和本科生产生积极影响。质谱法作为一种主要的生物分析工具的出现是仪器创新和新电离化学的产物，可以应用于复杂的生物分子。这项工作将在分析化学、生物化学和生物学领域产生重大影响。