Protein cross-linking of macromolecular complexes (A10)

我们将对静息和刺激的B细胞进行蛋白质交联结合质谱仪(MS)。关键信号分子的交联相互作用体将与B细胞受体(BCR)刺激时B细胞的磷酸化状态有关，并将获得有关磷酸化如何影响分子相互作用的信息。此外，我们将利用紫外光和化学交联剂在体外重组蛋白质-DNA复合体和体内、体外系统中建立蛋白质-DNA交联剂与MS相结合的方法。将获得染色质等DNA-蛋白质系统中的结构功能关系。

We will perform protein cross-linking combined with mass spectrometry (MS) of resting and stimulated B cells. The cross-linked interactome of key signaling molecules will be related to the phosphorylation states of the B cells upon B cell receptor (BCR) stimulation and information on how phosphorylation influences molecular interactions will be obtained. Further, we will establish protein-DNA cross-linking combined with MS of in vitro reconstituted protein-DNA complexes and in in vivo and ex vivo systems using UV-light and chemical cross-linker. Structural function relationships in DNA–protein systems like chromatin will be obtained.