Protein cross-linking of macromolecular complexes (A10)

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We will perform protein cross-linking combined with mass spectrometry (MS) of resting and stimulated B cells. The cross-linked interactome of key signaling molecules will be related to the phosphorylation states of the B cells upon B cell receptor (BCR) stimulation and information on how phosphorylation influences molecular interactions will be obtained. Further, we will establish protein-DNA cross-linking combined with MS of in vitro reconstituted protein-DNA complexes and in in vivo and ex vivo systems using UV-light and chemical cross-linker. Structural function relationships in DNA–protein systems like chromatin will be obtained.