IODP Microbiology Through Massively Parallel DNA Sequencing

通过大规模并行 DNA 测序进行 IODP 微生物学

• 批准号: 0550601
• 负责人: Christopher House
• 金额: --
• 依托单位: Pennsylvania State Univ University Park
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-02-15 至 2010-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0550601&HistoricalAwards=false
• 关键词: IODP; Microbiology; Through; Massively; Parallel

This program seeks to investigate the application of newly available, massively parallel DNA sequencing technology for the pursuit of environmental microbiology objectives, with particular attention to high-priority deep biosphere studies under IODP. Using 5 samples from Site 1229 of ODP Leg 201 (Peruvian deep biosphere drilling), the researchers will conduct a proof-of-concept study to sequence DNA focusing on material that is considered DNA-poor by conventional measures. They will first produce an estimated 300,000 sequences of 100 base pairs (~30 Mb) for each of the five samples and then map these metagenomes. During a second phase of the study, they will conduct a focused study either on an important horizon at Site 1229 or on samples from another hole and produce ~90 Mb of additional genomic DNA data in 3 or 4 rounds of sequencing. The primary broader impact of this research is its potential to revolutionize microbial ecology, particularly for difficult-to-study samples of the deep marine biosphere. In addition, the research will support a graduate student and strengthen the interdisciplinary collaboration between a geomicrobiologist and a microbial ecologist.

该计划旨在研究新的大规模并行DNA测序技术的应用，以实现环境微生物学目标，特别关注IODP下的高优先级深层生物圈研究。 使用ODP Leg 201（秘鲁深层生物圈钻探）1229号站点的5个样本，研究人员将进行概念验证研究，以DNA测序，重点关注传统措施认为DNA贫乏的材料。 他们首先将为五个样本中的每个样本产生估计300，000个包含100个碱基对（~30 Mb）的序列，然后绘制这些宏基因组。 在研究的第二阶段，他们将对1229号研究中心的重要水平线或另一个孔的样本进行重点研究，并在3或4轮测序中产生约90 Mb的额外基因组DNA数据。 这项研究的主要广泛影响是它有可能彻底改变微生物生态学，特别是对于难以研究的深海生物圈样本。 此外，该研究将支持一名研究生，并加强地质微生物学家和微生物生态学家之间的跨学科合作。