IODP Microbiology Through Massively Parallel DNA Sequencing

该计划旨在研究新出现的大规模平行DNA测序技术在环境微生物学目标中的应用，特别关注IODP下的高优先级深层生物圈研究。研究人员将利用ODP第201次钻探（秘鲁深层生物圈钻探）1229号地点的5个样本，进行概念验证研究，对传统方法认为DNA贫乏的材料进行DNA测序。他们将首先为五个样本中的每个样本产生大约30万个100个碱基对的序列（约30 Mb），然后绘制这些宏基因组。在研究的第二阶段，他们将在Site 1229的一个重要层位或另一个孔的样本上进行重点研究，并在3或4轮测序中产生约90mb的额外基因组DNA数据。这项研究的主要广泛影响是它有可能彻底改变微生物生态学，特别是对难以研究的深海生物圈样本。此外，这项研究将支持一名研究生，并加强一名地球微生物学家和一名微生物生态学家之间的跨学科合作。

This program seeks to investigate the application of newly available, massively parallel DNA sequencing technology for the pursuit of environmental microbiology objectives, with particular attention to high-priority deep biosphere studies under IODP. Using 5 samples from Site 1229 of ODP Leg 201 (Peruvian deep biosphere drilling), the researchers will conduct a proof-of-concept study to sequence DNA focusing on material that is considered DNA-poor by conventional measures. They will first produce an estimated 300,000 sequences of 100 base pairs (~30 Mb) for each of the five samples and then map these metagenomes. During a second phase of the study, they will conduct a focused study either on an important horizon at Site 1229 or on samples from another hole and produce ~90 Mb of additional genomic DNA data in 3 or 4 rounds of sequencing. The primary broader impact of this research is its potential to revolutionize microbial ecology, particularly for difficult-to-study samples of the deep marine biosphere. In addition, the research will support a graduate student and strengthen the interdisciplinary collaboration between a geomicrobiologist and a microbial ecologist.