Enhancing Food Testing Capabilities at the WVDA through Discipline A: Microbiology Analytical Track 5: Microbiology Capability / Capacity Development

实验室灵活资助模式FY2020 - 2021 西弗吉尼亚州农业部提案 PAR-20 - 105： LFFM（U19）不允许进行临床试验 学科A：微生物学 分析途径4：全基因组测序 摘要： 全基因组测序是一种实验室程序， （A，T，G和C）在一个过程中的生物体的基因组。小的dna片段 标签或条形码被添加到DNA中，以识别剪切的DNA片段 属于哪种细菌。来自多种细菌的条形码DNA被组合， 放入全基因组测序仪。测序仪识别组成 每个细菌序列，并使用条形码来跟踪哪些碱基属于 哪些细菌计算机分析工具可以用来比较细菌序列 并识别差异。差异的数量可以说明 细菌是什么，以及它们是同一爆发的一部分的可能性有多大。 全基因组测序是获取高水平信息的一种快速且经济实惠的方法 关于细菌的信息。将这一技术水平添加到 西弗吉尼亚州农业部的微生物学实验室将允许 部门，以获得细菌基因组数据在实时设置，并协助更快 全球范围内的疫情检测。 学科A的目的：微生物学;分析轨道4：全基因组 测序是为了增强西弗吉尼亚州内的GenomeTrakr网络 农业部监管和环境事务司通过捕获 FDA中非临床病原体的当前和不断发展的基因组多样性- 在人类或动物食品、环境和水样中的相关区域。的 通过这一分析轨道产生的测序数据将被公开保存， 国家生物技术信息中心（NCBI）的数据库，并将 供研究人员和公共卫生官员进行真实的时间比较， 分析将对食源性疾病暴发调查产生积极影响， 食源性疾病和死亡。

Laboratory Flexible Funding Model FY2020-2021 West Virginia Department of Agriculture Proposal PAR-20-105: LFFM (U19) Clinical Trials Not Allowed Discipline A: Microbiology Analytical Track 4: Whole Genome Sequencing Abstract: Whole genome sequencing is a laboratory procedure that determines the order of bases (A, T, G, and C) in the genome of an organism in one process. Small pieces of DNA tags, or bar codes, are added to the DNA to identify which piece of sheared DNA belongs to which bacteria. The bar-coded DNA from multiple bacteria are combined and put in the whole genome sequencer. The sequencer identifies the bases that make up each bacterial sequence and uses the bar code to keep track of which bases belong to which bacteria. Computer analysis tools can be used to compare bacterial sequences and identify differences. The number of differences can illustrate how closely related the bacteria are, and how likely it is that they are part of the same outbreak. Whole genome sequencing is a fast and affordable way to obtain high-level information about the bacteria using just one test. Adding this level of technology within the Microbiology laboratory at West Virginia Department of Agriculture will allow the department to obtain bacterial genomic data in a real-time setting and assist in faster outbreak detection on a global scale. The purpose of Discipline A: Microbiology; Analytical Track 4: Whole Genome Sequencing is to enhance the GenomeTrakr network within the West Virginia Department of Agriculture Regulatory and Environmental Affairs Division by capturing the current and evolving genomic diversity of non-clinical pathogens across FDA- relevant areas in human or animal food, environmental, and water samples. The sequencing data generated through this analytical track will be housed in public databases at the National Center for Biotechnology Information (NCBI) and will be accessed by researchers and public health officials for real time comparison and analysis that will positively impact foodborne illness outbreak investigations and reduce foodborne illnesses and death.