IDBR: Genemeter: A Portable DNA Identification Device

IDBR：Genemeter：便携式 DNA 识别设备

• 批准号: 0551990
• 负责人: Nader Pourmand
• 金额: $ 69.01万
• 依托单位: Stanford University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0551990&HistoricalAwards=false
• 关键词: IDBR; Genemeter; Portable; DNA; Identification

This award is for the development of a "Genemeter," a portable or desktop device for rapidly detecting and quantifying arrays of specific DNA targets at low concentrations. This instrument will use Charge-Perturbation Detection (CPD). CPD involves electronic detection with operational amplifiers circuitry, which lends itself to miniaturization of the device. The goal is to develop a user-friendly device that can also be used by non-experts, for example, in high school educational activities. A desktop version of the device with a 6x6 array will be developed by integrating a replaceable 6x6 electrode array sensor and an automated, computer-controlled reagent dispensing system. Combined with a desktop PC containing the appropriate hardware and software for signal data acquisition, analysis and user interface, this system will lay the groundwork for a more portable system developed at a later stage. The label-free electronic detection method integrated into the proposed multiple-electrode system will provide rapid and sensitive detection of genetic mutations and identification of DNA sequences in a sample. The CPD detection system is predicted to be superior to comparable detection systems in terms of speed, sensitivity, and cost. This will be a robust and effective biosensor for many molecular research applications and will constitute a major breakthrough in DNA-based biosensor technology. The Genemeter has the potential to profoundly influence the rapidly growing field of molecular diagnostics in both academic research and healthcare practice. Development of the Genemeter will be coupled with outreach in several ways. Device prototypes will be tested in graduate and undergraduate molecular biology courses at the university and at local Community Colleges. This wider exposure of the instrument will give more students an opportunity to learn about molecular biology and how the integration of multiple research fields can produce exciting new technologies. In addition, some of the discoveries in genomics and proteomics will be vividly displayed to high school students through the integration of this low-cost Genemeter into laboratory curricula, cultivating their scientific interests and helping to ensure the vitality of the people-power pipelines needed for the nation's scientific enterprise.

该奖项是为了开发一种“Genemeter”，一种便携式或台式设备，用于快速检测和定量低浓度的特定DNA靶点阵列。该仪器将使用电荷扰动检测（CPD）。CPD涉及使用运算放大器电路的电子检测，这有助于设备的小型化。我们的目标是开发一种用户友好的设备，也可以由非专家使用，例如，在高中教育活动。将通过集成可更换的6x6电极阵列传感器和自动化计算机控制的试剂分配系统来开发具有6x6阵列的设备的桌面版本。该系统与一台装有信号数据采集、分析和用户界面的适当硬件和软件的台式个人电脑相结合，将为以后开发一个更便携的系统奠定基础。集成到拟议的多电极系统中的无标记电子检测方法将提供快速、灵敏的基因突变检测和样本中DNA序列的识别。CPD检测系统被预测为在速度、灵敏度和成本方面优于可比检测系统的上级。这将是一个强大的和有效的生物传感器为许多分子研究应用，并将构成一个重大突破，在基于DNA的生物传感器技术。Genemeter有可能深刻影响学术研究和医疗实践中快速发展的分子诊断领域。Genemeter的开发将与几种方式的外联相结合。 设备原型将在大学和当地社区学院的研究生和本科生分子生物学课程中进行测试。仪器的这种更广泛的接触将使更多的学生有机会了解分子生物学以及多个研究领域的整合如何产生令人兴奋的新技术。此外，基因组学和蛋白质组学的一些发现将通过将这种低成本的Genemeter融入实验室课程生动地展示给高中生，培养他们的科学兴趣，并帮助确保国家科学事业所需的人力管道的活力。