IDBR: Genemeter: A Portable DNA Identification Device

IDBR：Genemeter：便携式 DNA 识别设备

• 批准号: 0830141
• 负责人: Nader Pourmand
• 金额: --
• 依托单位: University of California-Santa Cruz
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0830141&HistoricalAwards=false
• 关键词: IDBR; Genemeter; Portable; DNA; Identification

This award is for the development of a "Genemeter," a portable or desktop device for rapidly detecting and quantifying arrays of specific DNA targets at low concentrations. This instrument will use Charge-Perturbation Detection (CPD). CPD involves electronic detection with operational amplifiers circuitry, which lends itself to miniaturization of the device. The goal is to develop a user-friendly device that can also be used by non-experts, for example, in high school educational activities. A desktop version of the device with a 6x6 array will be developed by integrating a replaceable 6x6 electrode array sensor and an automated, computer-controlled reagent dispensing system. Combined with a desktop PC containing the appropriate hardware and software for signal data acquisition, analysis and user interface, this system will lay the groundwork for a more portable system developed at a later stage. The label-free electronic detection method integrated into the proposed multiple-electrode system will provide rapid and sensitive detection of genetic mutations and identification of DNA sequences in a sample. The CPD detection system is predicted to be superior to comparable detection systems in terms of speed, sensitivity, and cost. This will be a robust and effective biosensor for many molecular research applications and will constitute a major breakthrough in DNA-based biosensor technology. The Genemeter has the potential to profoundly influence the rapidly growing field of molecular diagnostics in both academic research and healthcare practice. Development of the Genemeter will be coupled with outreach in several ways. Device prototypes will be tested in graduate and undergraduate molecular biology courses at the university and at local Community Colleges. This wider exposure of the instrument will give more students an opportunity to learn about molecular biology and how the integration of multiple research fields can produce exciting new technologies. In addition, some of the discoveries in genomics and proteomics will be vividly displayed to high school students through the integration of this low-cost Genemeter into laboratory curricula, cultivating their scientific interests and helping to ensure the vitality of the people-power pipelines needed for the nation's scientific enterprise.

该奖项旨在表彰“Genemeter”的开发，这是一种便携式或台式设备，用于快速检测和定量低浓度特定 DNA 靶标阵列。该仪器将使用电荷扰动检测（CPD）。 CPD 涉及使用运算放大器电路的电子检测，这有助于设备的小型化。目标是开发一种用户友好的设备，非专家也可以使用，例如在高中教育活动中。该设备的桌面版本具有 6x6 阵列，将通过集成可更换的 6x6 电极阵列传感器和自动化、计算机控制的试剂分配系统来开发。该系统与包含用于信号数据采集、分析和用户界面的适当硬件和软件的台式PC相结合，将为后期开发更便携的系统奠定基础。集成到所提出的多电极系统中的无标记电子检测方法将提供快速、灵敏的基因突变检测和样本中 DNA 序列的识别。预计 CPD 检测系统在速度、灵敏度和成本方面优于同类检测系统。这将是一种适用于许多分子研究应用的强大而有效的生物传感器，并将构成基于 DNA 的生物传感器技术的重大突破。 Genemeter 有潜力对学术研究和医疗保健实践中快速发展的分子诊断领域产生深远影响。基因仪的开发将通过多种方式与推广相结合。 设备原型将在大学和当地社区学院的研究生和本科生分子生物学课程中进行测试。更广泛地接触该仪器将使更多学生有机会了解分子生物学以及多个研究领域的整合如何产生令人兴奋的新技术。此外，基因组学和蛋白质组学的一些发现将通过这种低成本基因仪融入实验室课程，生动地向高中生展示，培养他们的科学兴趣，有助于确保国家科学事业所需的人力管道的活力。