SGER: Impacts of Hurricane Katrina on Water Quality and Bacterial Communities in Southeastern Louisiana

SGER：卡特里娜飓风对路易斯安那州东南部水质和细菌群落的影响

• 批准号: 0553138
• 负责人: Michael LaMontagne
• 金额: $ 2.25万
• 依托单位: McNeese State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-10-15 至 2006-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0553138&HistoricalAwards=false
• 关键词: SGER; Impacts; Hurricane; Katrina; Water

0553138 LaMontague Hurricanes have long and lasting effects on the geology of coastal systems. Ecologists have also examined the effects of hurricanes on aquatic systems (Valiela et al., 1996; Paerl et al., 2003), but no previous studies have focused on bacterial dynamics that may occur in response to hurricanes. This proposal focuses on bacterial dynamics for basic ecological reasons, but also because of human health concerns. There are already signs that the bacterial responses are important and large in the aftermath of Hurricane Katrina. Especially there is a threat from infectious disease also includes the virulent pathogen, Vibrio vulnificans. This pathogen is endemic in the northern Gulf of Mexico (Kelly, 1982), and appears responsible for several fatalities in areas effected by Hurricane Katrina.In this study, we propose to sample the waters overlying and adjoining New Orleans, to quantify the effects of Hurricane Katrina on this critical system. We will analyze bacterial community structure by analysis of terminal restriction fragment polymorphisms (TRFLP) of PCR-amplified ribosomal genes. The TRFLPs we generate will allow us to identify the dominant bacteria in this system and quantify the relative contribution of feces to the microbiota. We will track human contamination signals, as it enters into the food web, by analysis of naturally occurring stable isotopes in particulate organic matter.

0553138拉蒙塔古飓风对沿海系统的地质有长期和持久的影响。生态学家还研究了飓风对水生系统的影响（Valiela等人，1996; Paerl等人，2003年），但没有以前的研究集中在细菌动力学，可能会发生在应对飓风。这项建议侧重于细菌动力学的基本生态原因，但也因为人类健康的关注。已经有迹象表明，在卡特里娜飓风过后，细菌的反应是重要和巨大的。特别是有威胁的传染病还包括致命的病原体，创伤弧菌。这种病原体是墨西哥湾北方的地方病（Kelly，1982年），似乎是卡特里娜飓风影响地区造成多人死亡的原因。在这项研究中，我们建议对新奥尔良上方和邻近的沃茨水域进行采样，以量化卡特里娜飓风对这个关键系统的影响。我们将通过末端限制性片段多态性分析来分析细菌群落结构 PCR扩增的核糖体基因的TRFLP。我们生成的TRFLPs将使我们能够识别该系统中的优势细菌，并量化粪便对微生物群的相对贡献。我们将通过分析颗粒有机物中天然存在的稳定同位素来跟踪人类污染信号，因为它进入了食物网。