Collaborative Research: RAPID: Characterization of Microbial Community Structure and Fecal Contamination of Floodwaters Generated by Hurricane Harvey
合作研究:RAPID:飓风哈维产生的洪水的微生物群落结构和粪便污染的表征
基本信息
- 批准号:1759542
- 负责人:
- 金额:$ 2.39万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2017
- 资助国家:美国
- 起止时间:2017-10-15 至 2018-05-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
1759542 (LaMontagne) and 1759540 (Gentry). Floodwaters generated by hurricanes and other extreme weather events may contain dangerous levels of microbial pathogens, associated with sewage and human waste contamination, and toxins. To determine the source of fecal contamination of floodwater and the impact of this floodwater on receiving water quality, the investigators will collect water samples at stations surrounding an estuary between Houston and Galveston. Samples include those already drawn before and also immediately after Hurricane Harvey deluged Houston. Analysis of early samples shows that microbial contamination is present post storm. The investigators will apply quantitative modern molecular biology methods to determine the source of the microbes that contaminated these floodwaters and will continue sampling weekly until the microbial community, as assessed by the modern methods, returns to pre-storm conditions.Little is known about the risk associated with exposure by contact to floodwaters or the impact of these floodwaters on receiving water quality. Fecal indicator bacteria (FIB), used to assess contact risk for recreational waters, do not determine the source of microbial contamination and assessing the health of receiving waters remains challenging. Quantitative PCR and metagenomic analysis can help track the source of microbial contamination but few studies have conducted these analyses, particularly with pre-flood samples for comparison. Further, few studies have applied next-generation sequencing methods, which have the depth and coverage to identify microbes that reflect fecal contamination and the overall microbial community diversity and structure. This work will allow the determination of the source of fecal contamination of floodwaters generated in this event and could lead to an improved understanding of the health risks associated with exposure to floodwaters in future events and the impact of hurricanes on the health of coastal systems.
1759542(拉蒙塔涅)和1759540(绅士)。飓风和其他极端天气事件产生的洪水可能含有与污水和人类排泄物污染有关的危险水平的微生物病原体和毒素。为了确定洪水粪便污染的来源以及洪水对接收水质的影响,调查人员将在休斯顿和加尔维斯顿之间的河口周围的站点采集水样。样本包括在飓风哈维淹没休斯顿之前和之后立即采集的样本。对早期样本的分析表明,风暴过后存在微生物污染。调查人员将应用定量的现代分子生物学方法来确定污染这些洪水的微生物的来源,并将继续每周采样,直到通过现代方法评估的微生物群落恢复到风暴前的状态。人们对接触洪水的风险或这些洪水对接收水质的影响知之甚少。用于评估娱乐水域接触风险的粪便指示菌(FIB)不确定微生物污染源,评估接收水的健康状况仍然具有挑战性。定量聚合酶链式反应和总基因组分析可以帮助追踪微生物污染的来源,但很少有研究进行这些分析,特别是与洪水前的样本进行比较。此外,很少有研究应用下一代测序方法,这种方法具有深度和覆盖面,可以识别反映粪便污染的微生物以及整个微生物群落的多样性和结构。这项工作将有助于确定这一事件产生的洪水的粪便污染源,并有助于更好地了解未来事件中暴露在洪水中的健康风险以及飓风对沿海系统健康的影响。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Michael LaMontagne其他文献
Fecal coliform loadings and stocks in buttermilk bay, Massachusetts, USA, and management implications
- DOI:
10.1007/bf02589625 - 发表时间:
1991-09-01 - 期刊:
- 影响因子:3.000
- 作者:
Ivan Valiela;Merryl Alber;Michael LaMontagne - 通讯作者:
Michael LaMontagne
Michael LaMontagne的其他文献
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{{ truncateString('Michael LaMontagne', 18)}}的其他基金
Equipment: MRI: Track 1 Acquisition of a MALDI-TOF/FTIR System to Support Course-Based Undergraduate Research Experiences in Biological and Computational Sciences at an HSI
设备: MRI:轨道 1 采购 MALDI-TOF/FTIR 系统,以支持 HSI 生物和计算科学方面基于课程的本科生研究经验
- 批准号:
2320765 - 财政年份:2023
- 资助金额:
$ 2.39万 - 项目类别:
Standard Grant
RAPID: A Course-based Undergraduate Research Experience on the Interaction between the Nasal Microbiome and SARS-CoV-2 Coronavirus Infection
RAPID:基于课程的本科生关于鼻微生物组与 SARS-CoV-2 冠状病毒感染之间相互作用的研究经验
- 批准号:
2028400 - 财政年份:2020
- 资助金额:
$ 2.39万 - 项目类别:
Standard Grant
SGER: Impacts of Hurricane Katrina on Water Quality and Bacterial Communities in Southeastern Louisiana
SGER:卡特里娜飓风对路易斯安那州东南部水质和细菌群落的影响
- 批准号:
0553138 - 财政年份:2005
- 资助金额:
$ 2.39万 - 项目类别:
Standard Grant
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