Systemic acquired resistance in plants - an omics approach to pathogen defence signalling

植物的系统获得性抗性——病原体防御信号的组学方法

• 批准号: 175245521
• 负责人: Professorin Dr. A. Corina Vlot-Schuster
• 金额: --
• 依托单位: Professur Genetik der Nutzpflanzen
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/175245521?language=en
• 关键词: Systemic; acquired; resistance; plants; omics

Immunity in plants is based in part on recognition of pathogen effector molecules by corresponding plant RESISTANCE (R) gene products. This type of immunity is accompanied by the induction of a long-lasting broad spectrum defence, systemic acquired resistance (SAR), in the remaining healthy tissues of the plant. The SAR state is maintained at low energy cost, and SAR signalling components could be used to protect crops from disease without significant loss of yield. Therefore, we aim to identify SAR signalling components that affect the generation and/or transmission of mobile SAR signals. To this end, we will use the model plant Arabidopsis thaliana and a mutant, eds1, which is incapable of generating or transmitting SAR signals in response to the bacterial effector AvrRpm1. First, the peptide, lipid, and polar small molecule content of extracts of AvrRpm1-expressing wild type (wt) as compared to eds1 plants will be analyzed by different mass spectrometry-based techniques. Second, the defence-inducing potential of thus identified candidate SAR signalling compounds will be examined in A. thaliana and other plant species, including the crop plant tomato. Finally, we will study how new SAR signals affect other regulatory processes, e.g. those affecting drought stress tolerance, by transcript profiling.

植物中的免疫部分基于相应的植物抗性（R）基因产物对病原体效应分子的识别。这种类型的免疫伴随着在植物的剩余健康组织中诱导持久的广谱防御，系统获得性抗性（SAR）。SAR状态以低能量成本维持，并且SAR信号成分可用于保护作物免受疾病的影响而不会显著损失产量。因此，我们的目标是确定SAR信令组件，影响生成和/或传输的移动的SAR信号。为此，我们将使用模式植物拟南芥和突变体，eds1，这是无法产生或传输SAR信号响应细菌效应AvrRpm 1。首先，将通过不同的基于质谱的技术分析AvrRpm1表达野生型（wt）与eds1植物相比的提取物的肽、脂质和极性小分子含量。第二，将在A中检查由此鉴定的候选SAR信号传导化合物的防御诱导潜力。thaliana和其他植物物种，包括作物番茄。最后，我们将研究新的SAR信号如何影响其他监管过程，例如，那些影响干旱胁迫耐受性，转录谱。