ME: Collaborative Research: Metabolic Engineering of E. coli Sugar-Utilization Regulatory Systems for the Consumption of Plant Biomass Sugars

ME:合作研究:大肠杆菌糖利用调节系统消耗植物生物质糖的代谢工程

基本信息

  • 批准号:
    0601549
  • 负责人:
  • 金额:
    $ 23.24万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Continuing Grant
  • 财政年份:
    2005
  • 资助国家:
    美国
  • 起止时间:
    2005-08-01 至 2007-08-31
  • 项目状态:
    已结题

项目摘要

The overall objective of this project is to metabolically engineer the E. coli Sugar-Utilization Regulatory Systems (SURS) to utilize sugar mixtures obtained from plant biomass. The Principal Investigators (PIs) will introduce specific genetic modifications to the SURS and analyze the consequences of these changes at the cellular level. The specific goals are to: (1) construct E. coli strains with modified SURS and evaluate their capability to efficiently utilize sugar mixtures (glucose, xylose and arabinose); (2) evaluate changes in the regulation of gene expression at the genomic scale resulting from engineering E. coli SURS; (3) identify functional metabolic pathways and quantify their fluxes in SURS mutants and wild type strains by using a novel flux analysis technique based on bondomer analysis of 2D NMR bond-labeling experiments; (4) integrate these results using a novel genetic network-based metabolic flux analysis (MFA) model that combines gene expression and metabolic flux data; and (5) propose further genetic/environmental modification for improving the capacity of SURS mutants for fermenting sugar mixtures. The benefits from this project will be two-fold. First, from an applied standpoint, the project will provide a powerful tool for designing of rational modifications of the SURS to obtain recombinant strains capable of efficiently metabolizing sugar mixtures. Second, from a fundamental standpoint, this project will contribute to a better understanding of the role of SURS in regulating both sugar utilization and several other cellular functions.
该项目的总体目标是代谢工程大肠杆菌糖利用调节系统(SURS)利用从植物生物质中获得的糖混合物。首席研究员(pi)将引入特定的遗传修饰的SURS,并在细胞水平上分析这些变化的后果。具体目标是:(1)构建带有修饰的sers的大肠杆菌菌株,并评估其有效利用糖混合物(葡萄糖、木糖和阿拉伯糖)的能力;(2)在基因组尺度上评估改造大肠杆菌链球菌后基因表达调控的变化;(3)利用基于二维核磁共振键标记实验的键聚体分析的新型通量分析技术,确定了苏氏突变体和野生型菌株的功能代谢途径并量化其通量;(4)利用结合基因表达和代谢通量数据的基于遗传网络的代谢通量分析(MFA)模型对这些结果进行整合;(5)提出进一步的遗传/环境改造,以提高SURS突变体发酵糖混合物的能力。这个项目的好处将是双重的。首先,从应用的角度来看,该项目将为设计合理的SURS修饰以获得能够有效代谢糖混合物的重组菌株提供强大的工具。其次,从基础的角度来看,该项目将有助于更好地理解SURS在调节糖利用和其他几种细胞功能中的作用。

项目成果

期刊论文数量(0)
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会议论文数量(0)
专利数量(0)

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Ramon Gonzalez其他文献

Sudden Fiction Latino: Short Short Stories from the U.S. and Latin America
拉丁裔突发小说:来自美国和拉丁美洲的短篇小说
  • DOI:
  • 发表时间:
    2010
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Ramon Gonzalez;Robert Shapard;James Thomas
  • 通讯作者:
    James Thomas
A new methodology to obtain wine yeast strains overproducing mannoproteins
  • DOI:
    10.1016/j.ijfoodmicro.2010.02.014
  • 发表时间:
    2010-04-30
  • 期刊:
  • 影响因子:
  • 作者:
    Manuel Quirós;Daniel Gonzalez-Ramos;Laura Tabera;Ramon Gonzalez
  • 通讯作者:
    Ramon Gonzalez
Designing artificial pathways for improving chemical production
设计用于改进化学生产的人工途径
  • DOI:
    10.1016/j.biotechadv.2023.108119
  • 发表时间:
    2023-05-01
  • 期刊:
  • 影响因子:
    12.500
  • 作者:
    Zaigao Tan;Jian Li;Jin Hou;Ramon Gonzalez
  • 通讯作者:
    Ramon Gonzalez
Scavenging intracellular reactive oxygen species to boost methanol assimilation
清除细胞内的活性氧以促进甲醇同化作用
  • DOI:
    10.1016/j.cej.2025.164002
  • 发表时间:
    2025-07-15
  • 期刊:
  • 影响因子:
    13.200
  • 作者:
    Haiyan Liu;Yun Chen;Jian Li;Cheng Zhu;Jiahui Peng;Ramon Gonzalez;Yanfen Bai;Zaigao Tan
  • 通讯作者:
    Zaigao Tan
Anaerobic fermentation of glycerol: a platform for renewable fuels and chemicals
  • DOI:
    10.1016/j.tibtech.2012.10.006
  • 发表时间:
    2013-01-01
  • 期刊:
  • 影响因子:
  • 作者:
    James M. Clomburg;Ramon Gonzalez
  • 通讯作者:
    Ramon Gonzalez

Ramon Gonzalez的其他文献

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{{ truncateString('Ramon Gonzalez', 18)}}的其他基金

IPA Agreement
国际投资协定协议
  • 批准号:
    2152426
  • 财政年份:
    2021
  • 资助金额:
    $ 23.24万
  • 项目类别:
    Intergovernmental Personnel Award
Collaborative Research: Understanding and exploiting the structure-function link between fatty acid biosynthesis and degradation enzymes for functionalized small molecule synthesis
合作研究:了解和利用脂肪酸生物合成和功能化小分子合成的降解酶之间的结构功能联系
  • 批准号:
    1805793
  • 财政年份:
    2018
  • 资助金额:
    $ 23.24万
  • 项目类别:
    Standard Grant
SusChEM: Collaborative Research: Efficient biological activation and conversion of short-chain hydrocarbons
SusChEM:合作研究:短链碳氢化合物的高效生物活化和转化
  • 批准号:
    1706120
  • 财政年份:
    2017
  • 资助金额:
    $ 23.24万
  • 项目类别:
    Standard Grant
Universal platform for the bioconversion of single carbon feedstocks to fuels and chemicals
用于将单一碳原料生物转化为燃料和化学品的通用平台
  • 批准号:
    1605999
  • 财政年份:
    2016
  • 资助金额:
    $ 23.24万
  • 项目类别:
    Standard Grant
MRI: Acquisition of Analytical Instrumentation for a State-of-the-Art Proteomic Facility at Rice University
MRI:为莱斯大学最先进的蛋白质组学设施购置分析仪器
  • 批准号:
    0723039
  • 财政年份:
    2007
  • 资助金额:
    $ 23.24万
  • 项目类别:
    Standard Grant
CAREER: Understanding and Harnessing the Fermentative Metabolism of Glycerol in E. coli: A New Path to Biofuels and Biochemicals
职业:了解和利用大肠杆菌中甘油的发酵代谢:生物燃料和生物化学品的新途径
  • 批准号:
    0645188
  • 财政年份:
    2007
  • 资助金额:
    $ 23.24万
  • 项目类别:
    Continuing Grant
ME: Collaborative Research: Metabolic Engineering of E. coli Sugar-Utilization Regulatory Systems for the Consumption of Plant Biomass Sugars
ME:合作研究:大肠杆菌糖利用调节系统消耗植物生物质糖的代谢工程
  • 批准号:
    0331388
  • 财政年份:
    2003
  • 资助金额:
    $ 23.24万
  • 项目类别:
    Continuing Grant

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