SusChEM: Collaborative Research: Efficient biological activation and conversion of short-chain hydrocarbons

SusChEM：合作研究：短链碳氢化合物的高效生物活化和转化

• 批准号: 1706120
• 负责人: Ramon Gonzalez
• 金额: $ 20万
• 依托单位: William Marsh Rice University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-07-01 至 2019-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1706120&HistoricalAwards=false
• 关键词: SusChEM; Collaborative; Research; Efficient; biological

Advances in the recovery of oil and gas reserves, through the use of fracking and horizontal well drilling, for example, have dramatically increased the amount of natural gas that can be economically harvested in the US. These reserves also contain compounds, know as natural gas liquids (NGLs), that are expensive to convert to useful products using current refinery technology. Fortunately, many microorganisms are capable of consuming NGLs for biomass and energy production using pathways that are much more efficient and less expensive. The biological mechanisms of these conversions will be studied in order to design organisms that exhibit even greater yield of product. By eliminating the generation of waste products and enhancing the effectiveness of these microbes, the resulting technology will be more widely deployed at fracking sites, increasing the energy security of the US while decreasing the negative environmental impact of natural gas extraction. This project will also provide STEM research opportunities to traditionally underrepresented groups, including to high school students through outreach to high schools in Houston, through the Science Academy of South Texas, and through the McNair program at Penn State.By cloning and repurposing poorly understood alkane-metabolizing enzymes, this project aims to develop metabolically engineered microorganisms capable of converting short-chain alkanes to target products such as butanol. Harnessing alkylsuccinate synthases for such biocatalytic applications requires functionally expressing their partner "activase" enzymes, that require iron-sulfur cluster incorporation, in a microbial host amenable to metabolic engineering. Screens for functional expression of known or putative enzyme pairs and for activation by non-cognate activases will be executed. Enzyme directed evolution strategies to achieve alkylsuccinate synthase activation will also be employed. Functional, heterologous partners will be thoroughly characterized, providing new insights into activase-alkylsuccinate synthase interaction, and new opportunities for biotechnological applications of these and related enzymes. The overall research plan combines protein and metabolic engineering to establish a novel engineering platform for biocatalytic processes with enhanced carbon and energy efficiencies. If successful, this project could transform the commercial processing of NGL away from large, capital- and energy-intensive refinery processing to smaller-scale processes with high mass and energy efficiency. This would in turn eliminate a major source of waste involved in natural gas processing.

例如，通过使用水力压裂法和水平井钻井，石油和天然气储量的开采取得了进步，这极大地增加了美国可经济开采的天然气量。这些储量还含有被称为天然气液体（ngl）的化合物，使用当前的炼油技术将其转化为有用的产品是昂贵的。幸运的是，许多微生物能够利用效率更高、成本更低的途径消耗ngl来生产生物质和能源。这些转化的生物学机制将被研究，以便设计出表现出更高产量的生物。通过消除废物的产生并提高这些微生物的有效性，由此产生的技术将更广泛地应用于水力压裂现场，提高美国的能源安全，同时减少天然气开采对环境的负面影响。该项目还将为传统上代表性不足的群体提供STEM研究机会，包括通过与休斯顿高中、南德克萨斯科学院和宾夕法尼亚州立大学麦克奈尔项目的联系，为高中生提供STEM研究机会。通过克隆和重新利用尚不清楚的烷烃代谢酶，该项目旨在开发能够将短链烷烃转化为丁醇等目标产品的代谢工程微生物。利用烷基琥珀酸合成酶进行这种生物催化应用，需要在微生物宿主中表达它们的伙伴“激活酶”，这需要铁硫簇结合，从而适应代谢工程。筛选已知或假定的酶对的功能表达和非同源激活酶的激活。酶定向进化策略，以实现烷基琥珀酸合酶激活也将采用。功能异源合作伙伴将被彻底表征，为活化酶-烷基琥珀酸合酶的相互作用提供新的见解，并为这些酶和相关酶的生物技术应用提供新的机会。总体研究计划将蛋白质和代谢工程相结合，为提高碳和能源效率的生物催化过程建立一个新的工程平台。如果成功，该项目将使液化天然气的商业加工从大型、资本和能源密集型的炼油厂加工转变为规模较小、质量高、能源效率高的加工。这反过来又会消除天然气加工过程中的一个主要废物来源。