SBIR Phase I: Rapid Detection of Whole Pathogens with Microchannel Resonators

SBIR 第一阶段：使用微通道谐振器快速检测整个病原体

• 批准号: 0610933
• 负责人: Ken Babcock
• 金额: $ 10万
• 依托单位: Innovative Micro Technology
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2006-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0610933&HistoricalAwards=false
• 关键词: SBIR; Phase; Rapid; Detection; Whole

This Small Business Innovation Research Phase 1 project will test the feasibility of a new detector of whole pathogenic organisms that is sensitive enough to detect a single anthrax spore, more rapid than polymerase chain reaction (PCR), and as specific as established assays based on biomolecular affinity. The detector is the suspended microchannel resonator (SMR), a recently developed MEMs-based sensor that measures mass in fluid with unprecedented precision. This project will fabricate SMR sensors that have been modified to accept whole pathogens up to micron-sized spores, and functionalize them using antibody and peptide receptors. The detectors sensitivity, specificity, and throughput will be tested on anthrax and M13 bacteriophages. The results will help determine whether the SMR performance may ultimately surpass the current state of theart for detection of biowarfare agents or infectious disease.Such a product may help satisfy the great need for rapid detection of bioterrorism agents and accelerated diagnosis in clinical settings. For the case of weaponized anthrax, it may be possible to identify specific spores in minutes, from eluted nasal swabs and other sources with low spore count. By providing actionable information in minutes or hours, the SMR would help fill the current gap between local screening of anthrax exposure, and confirmatory testing which currently takes days.

这个小型企业创新研究第一阶段项目将测试一种新型完整病原生物检测器的可行性，该检测器足够灵敏，可以检测单个炭疽孢子，比聚合酶链式反应 (PCR) 更快，并且与基于生物分子亲和力的现有检测方法一样具体。该探测器是悬浮式微通道谐振器 (SMR)，这是一种最近开发的基于 MEM 的传感器，能够以前所未有的精度测量流体中的质量。该项目将制造经过修改的 SMR 传感器，可以接受微米大小孢子的整个病原体，并使用抗体和肽受体对其进行功能化。检测器的灵敏度、特异性和通量将在炭疽和 M13 噬菌体上进行测试。结果将有助于确定SMR性能是否最终可能超越目前检测生物战剂或传染病的最先进水平。这样的产品可能有助于满足临床环境中快速检测生物恐怖剂和加速诊断的巨大需求。对于武器化炭疽病例，可以在几分钟内从洗脱的鼻拭子和其他孢子数低的来源中识别出特定的孢子。通过在几分钟或几小时内提供可操作的信息，SMR 将有助于填补目前本地炭疽暴露筛查与目前需要数天时间的确认测试之间的差距。