SBIR Phase I: Rapid, End-to-end Sample Preparation for Sequencing Applications

SBIR 第一阶段：用于测序应用的快速、端到端样品制备

• 批准号: 2222688
• 负责人: Ryan Montes
• 金额: $ 27.42万
• 依托单位: MICROPURE GENOMICS INC.
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-01-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2222688&HistoricalAwards=false
• 关键词: SBIR; Phase; Rapid; End; end

The broader impact/commercial potential of this Small Business Innovation Research (SBIR) Phase I project consists of advancing methods for preparing deoxyribonucleic acid (DNA) for sequencing. Prior to sequencing a biological sample, DNA must be liberated from cells and separated from proteins and other unwanted debris, and then mixed with specialty buffers and chemical agents. This skilled task is currently carried out by trained scientists using largely manual manipulations of the samples and expensive equipment. The alternative method proposed in this project will speed-up diagnoses from genomic sequencing by significantly reducing preparation time while also making preparation more reliable through automation. Notably, the proposed approach is expected to prepare DNA without reducing its length; consequently, the process should be ideally suited for preparing samples for emerging long-read sequencing technologies. These improvements have the potential to decrease the burden and costs associated with DNA sequencing, hence expanding the benefits of DNA sequencing technology to wider segments of society.This Small Business Innovation Research (SBIR) Phase I project relies upon a process for trapping genomic material in a small flow cell through which an electric field and pressure-driven flow are simultaneously applied. The process is highly selective towards strands of DNA or (ribonucleic acid) RNA; proteins and other, unwanted debris that enters the flow cell passes through. Also, the process is relatively gentle, so the length of sample should not be shortened as a result. This project will advance the technology to the marketplace by: (1) Completing cartridge design details and fabrication, including evaluation of material options; (2) Building a custom research and development platform for interfacing with the cartridges; (3) Developing methods for DNA sample extraction and transfer; (4) Developing the library preparation protocols using mixing and heating while toggling the Electro-Hydrodynamic Trapping; and, (5) Integrating the entirety of the preparation process into a single cartridge and validating the process performance.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

小型企业创新研究(SBIR)第一阶段项目的更广泛影响/商业潜力包括先进的制备脱氧核糖核酸(DNA)用于测序的方法。在对生物样本进行测序之前，DNA必须从细胞中解放出来，从蛋白质和其他不需要的碎片中分离出来，然后与特殊的缓冲液和化学试剂混合。目前，这项熟练的任务是由训练有素的科学家进行的，他们主要使用人工操作样本和昂贵的设备。本项目中提出的替代方法将通过显著减少准备时间，同时通过自动化使准备更加可靠，从而加快从基因组测序进行诊断的速度。值得注意的是，拟议的方法有望在不缩短DNA长度的情况下制备DNA；因此，该过程应该非常适合于为新兴的长阅读测序技术准备样本。这些改进有可能减轻与DNA测序相关的负担和成本，从而将DNA测序技术的好处扩大到更广泛的社会阶层。这个小型企业创新研究(SBIR)第一阶段项目依赖于一种将基因组材料捕获到小流动细胞中的过程，通过该过程同时施加电场和压力驱动流动。这一过程对DNA或(核糖核酸)RNA链具有高度的选择性；进入流动细胞的蛋白质和其他不需要的碎片通过。此外，过程相对温和，因此样品长度不应因此而缩短。该项目将通过以下方式将技术推向市场：(1)完成试剂盒的设计细节和制造，包括对材料选项的评估；(2)建立与试剂盒接口的定制研究和开发平台；(3)开发DNA样本提取和转移的方法；(4)开发使用混合和加热的文库制备方案，同时切换电流体捕集；以及(5)将整个制备过程集成到一个试剂盒中，并验证过程性能。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，认为值得支持。