Protein Electron Transfer: Gating and Dynamics.

蛋白质电子转移：门控和动力学。

• 批准号: 0616656
• 负责人: Bruce Bowler
• 金额: $ 37.5万
• 依托单位: University of Denver
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0616656&HistoricalAwards=false
• 关键词: Protein; Electron; Transfer; Gating; Dynamics.

This award in the Inorganic, Bioinorganic and Organometallic Chemistry program supports research by Professor Bruce E. Bowler at the University of Denver to investigate fundamental aspects of gating and dynamics of electron transfer (ET) reactions in proteins. The model system to be investigated is based on the alkaline conformer of cytochrome c (Cytc). The rate of ET gating and the rate of the conformational change involved in gating will be measured independently and the thermodynamics of the conformational change will be assessed. Rates of gated ET in the iso-1-Cytc model system, will be determined by (1) varying the position of the histidine ligand in the surface loop unfolded by the alkaline conformer as a means of extending the range of gating rates available, (2) introducing substitutions for Phe 82 to fine-tune the 100 ms gating rate of the His 73-heme alkaline conformer and (3) replacing Pro 76 with Gly to simplify ET gating kinetics. The effect on electronic coupling by mutations at positions 52 and 67, which modulate the dynamics of the surface loop running from positions 40 to 57 in Cytc will also be probed. Substrates for photoinducible gated ET in the iso-1-Cytc model system will be developed. These studies will provide fundamental insight into the factors that affect electron transfer in proteins and practical strategies for protein-based molecular electronics devices. The research provides undergraduate and graduate students with independent forefront research projects. Consequently, the students trained in this project will be primed to move toward the emerging field of molecular electronics.

这个奖项在无机，生物无机和有机化学计划支持研究教授布鲁斯E。Bowler博士在丹佛大学研究蛋白质中电子转移（ET）反应的门控和动力学的基本方面。 待研究的模型系统是基于细胞色素c（Cytc）的碱性构象。 将独立测量ET门控速率和门控中涉及的构象变化速率，并评估构象变化的热力学。iso-1-Cytc模型系统中门控ET的速率将通过以下方式确定：（1）改变组氨酸配体在由碱性构象异构体展开的表面环中的位置，作为扩展可用门控速率范围的手段，（2）引入Phe 82的取代以微调His 73-血红素碱性构象体的100 ms门控速率，以及（3）用Gly代替Pro 76以简化ET门控动力学。 还将探讨位置52和67处的突变对电子偶联的影响，这些突变调节Cytc中从位置40至57运行的表面环的动力学。 将开发用于iso-1-Cytc模型系统中的光诱导门控ET的底物。这些研究将为蛋白质电子转移的影响因素和基于蛋白质的分子电子器件的实用策略提供基本的见解。 该研究为本科生和研究生提供了独立的前沿研究项目。 因此，在这个项目中训练的学生将准备向新兴的分子电子学领域迈进。